实时 PCR 检测法用于检测新型 Ib 分支猴痘病毒，时间为 2023 年 9 月至 2024 年 5 月。
Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024.
机构信息
Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands.
These authors contributed equally to this work and share first authorship.
出版信息
Euro Surveill. 2024 Aug;29(32). doi: 10.2807/1560-7917.ES.2024.29.32.2400486.
Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.
猴痘病毒（MPXV）是一种新兴的人畜共患病原体，具有复杂的流行病学特征，需要快速诊断并区分其分支和亚分支。新兴的 Ib 分支缺乏疾病控制和预防中心用于 I 分支特异性检测的基因组区域。我们报告了一种可特异性检测 Ib 分支的 MPXV 实时 PCR 方法。该方法在 92 个样本中表现出良好的灵敏度和特异性，可与其他 TaqMan 基检测方法一起用于检测 MPXV 并区分分支和亚分支。
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