Faculty of Chemical Engineering and Technology, Institute of Chemistry and Inorganic Technology, Cracow University of Technology, Warszawska St. 24, Cracow 31-155, Poland.
J Phys Chem B. 2024 Aug 22;128(33):8007-8016. doi: 10.1021/acs.jpcb.4c02526. Epub 2024 Aug 9.
Nanozymes are nanoscale materials that exhibit enzymatic-like activity, combining the benefits of nanomaterials with biocatalytic effects. The addition of metals to nanomaterials can enhance their nanozyme activity by mimicking the active sites of enzymes, providing structural support and promoting redox activity. In this study, nanostructured oxide and silicate-phosphate nanomaterials with varying manganese and copper additions were characterized. The objective was to assess the influence of metal modifications (Mn and Cu) on the acquisition of the nanozymatic activity by selected nanomaterials. An increase in manganese content in each material enhanced proteolytic activity (from 20 to 40 mUnit/mg for BG-Mn), while higher copper addition in glassy materials increased activity by 40%. Glassy materials exhibited approximately twice the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical activity (around 40 μmol/mL) compared to that of oxide materials. The proteolytic and antioxidant activities discussed in the study can be considered indicators for evaluating the enzymatic properties of the nanomaterials. Observations conducted on nanomaterials may aid in the development of materials with enhanced catalytic efficiency and a wide range of applications.
纳米酶是具有酶样活性的纳米级材料，将纳米材料的优点与生物催化效应相结合。在纳米材料中添加金属可以模拟酶的活性位点，提供结构支撑并促进氧化还原活性，从而增强其纳米酶活性。在这项研究中，对具有不同锰和铜添加量的纳米结构氧化物和硅酸盐-磷酸盐纳米材料进行了表征。目的是评估金属修饰（Mn 和 Cu）对所选纳米材料获得纳米酶活性的影响。每种材料中锰含量的增加都增强了蛋白水解活性（BG-Mn 从 20 增加到 40 mUnit/mg），而玻璃状材料中较高的铜添加量使活性增加了 40%。玻璃状材料的 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基活性（约 40 μmol/mL）约为氧化物材料的两倍。本研究中讨论的蛋白水解和抗氧化活性可被视为评估纳米材料酶性质的指标。对纳米材料的观察可能有助于开发具有增强催化效率和广泛应用的材料。
