Geng Jingzhang, Wu Yunxia, Tian Honglei, Dong Jianwei
Shaanxi Province Key Laboratory of Bio-Resources, QinLing-Bashan Mountains Bioresources Comprehensive Development C. I. C., Qinba State Key Laboratory of Biological Resources and Ecological Environment, Shaanxi University of Technology, 1 East 1st Ring Road, Hanzhong 723001, China.
School of Life Science and Technology, Xi'an Jiaotong University, Xianning West Road, Xi'an 710049, China.
Foods. 2024 Jul 26;13(15):2360. doi: 10.3390/foods13152360.
Hyperlipidemia is a chronic disease that is difficult to cure, and long-term pharmacotherapy may have negative consequences. Dietary therapy is a very promising strategy, and Chinese rice wine (Huangjiu) will play an important role because of its many biologically active components. In this work, the alleviating effect of Miq. Huangjiu (CSCHJ) on high-fat diet-induced hyperlipidemia in mice was investigated, which is brewed from the wheat Qu with the addition of Miq. and contains 15.54 g/L of polysaccharides. The experimental results showed that CSCHJ inhibited appetite, reduced body weight and blood sugar levels, and downregulated the serum levels of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) while concurrently upregulating high-density lipoprotein cholesterol (HDL-C) in the high-fat diet-induced hyperlipidemia mice. At the same time, it was discovered that alcohol worsens hyperlipidemia symptoms and related physiological markers, implying that CSCHJ polysaccharides may play a role in hyperlipidemia treatment. Through the assessment of organ indices, liver and kidney function, and tissue staining, CSCHJ demonstrated efficacy in repairing liver, kidney, and colon mucosal damage in hyperlipidemic mice. Furthermore, 16S rDNA sequencing and gas chromatography studies revealed that CSCHJ effectively restored the intestinal microbial structure and enhanced the quantity of fecal short-chain fatty acids (SCFAs) in hyperlipidemic mice. Therefore, the alleviating effect of CSCHJ on hyperlipidemia in mice may be attributed to its regulation of energy metabolism by repairing liver, kidney, and colon mucosal damage and restoring the gut microbiota structure, among other mechanisms. Overall, our findings provide evidence that CSCHJ contains active ingredients capable of alleviating hyperlipidemia, thereby laying a theoretical foundation for the extraction of bioactive substances from Huangjiu for future medical or dietary use.
高脂血症是一种难以治愈的慢性疾病，长期药物治疗可能会产生负面后果。饮食疗法是一种非常有前景的策略，而中国黄酒因其多种生物活性成分将发挥重要作用。在这项工作中，研究了添加了米曲酿造而成、含有15.54 g/L多糖的米曲黄酒（CSCHJ）对高脂饮食诱导的小鼠高脂血症的缓解作用。实验结果表明，CSCHJ抑制食欲，降低体重和血糖水平，下调高脂饮食诱导的高脂血症小鼠血清中的总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）水平，同时上调高密度脂蛋白胆固醇（HDL-C）水平。同时，发现酒精会加重高脂血症症状和相关生理指标，这意味着CSCHJ多糖可能在高脂血症治疗中发挥作用。通过对器官指数、肝肾功能和组织染色的评估，CSCHJ在修复高脂血症小鼠的肝、肾和结肠黏膜损伤方面显示出疗效。此外，16S rDNA测序和气相色谱研究表明，CSCHJ有效恢复了高脂血症小鼠的肠道微生物结构，并增加了粪便短链脂肪酸（SCFA）的含量。因此CSCHJ对小鼠高脂血症的缓解作用可能归因于其通过修复肝、肾和结肠黏膜损伤以及恢复肠道微生物群结构等机制来调节能量代谢。总体而言，我们的研究结果证明CSCHJ含有能够缓解高脂血症的活性成分，从而为未来从黄酒中提取生物活性物质用于医学或饮食用途奠定了理论基础。
