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基于转录组学和代谢组学分析探究提取物在多菌灵诱导的PC12细胞损伤模型中的抗氧化机制

Investigation into Antioxidant Mechanism of Extract in Carbendazim-Induced PC12 Cell Injury Model through Transcriptomics and Metabolomics Analyses.

作者信息

Liu Pingxiang, Chen Ju, Wen Xing, Shi Xin, Yin Xiaoqian, Yu Jiang, Qian Yongzhong, Gou Chunlin, Xu Yanyang

机构信息

Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

出版信息

Foods. 2024 Jul 28;13(15):2384. doi: 10.3390/foods13152384.

DOI:10.3390/foods13152384
PMID:39123576
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11311554/
Abstract

L., an important functional food in China, has antioxidant and antiaging activity. However, the exact antioxidant activity mechanism of extracts (LBE) is not well understood. Therefore, a carbendazim (CBZ)-induced PC12 cell injury model was constructed and vitrificated to study the antioxidant activity of fresh LBE on the basis of extraction parameter optimization via the full factorial design of experiments (DOE) method. The results showed that the pretreatment of PC12 cells with LBE could reduce the reactive oxygen species (ROS) level by 14.6% and inhibited the mitochondrial membrane potential (MMP) decline by 12.0%. Furthermore, the integrated analysis revealed that LBE played an antioxidant role by activating oxidative phosphorylation (OXPHOS) and restoring MMP, maintaining the tricarboxylic acid (TCA) cycle stability, and regulating the GSH metabolic pathway. The results of the present study provide new ideas for the understanding of the antioxidant function of LBE from a global perspective.

摘要

在中国，L是一种重要的功能性食品，具有抗氧化和抗衰老活性。然而，提取物（LBE）的确切抗氧化活性机制尚不清楚。因此，构建并验证了多菌灵（CBZ）诱导的PC12细胞损伤模型，以通过全因子实验设计（DOE）方法在优化提取参数的基础上研究新鲜LBE的抗氧化活性。结果表明，用LBE预处理PC12细胞可使活性氧（ROS）水平降低14.6%，并抑制线粒体膜电位（MMP）下降12.0%。此外，综合分析表明，LBE通过激活氧化磷酸化（OXPHOS）和恢复MMP、维持三羧酸（TCA）循环稳定性以及调节谷胱甘肽（GSH）代谢途径发挥抗氧化作用。本研究结果为从全局角度理解LBE的抗氧化功能提供了新思路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/c5e355d03452/foods-13-02384-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/07ceb3566b65/foods-13-02384-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/06d5d3d3ac58/foods-13-02384-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/39aa30d614ab/foods-13-02384-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/b83f5785009d/foods-13-02384-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/83d2766a630c/foods-13-02384-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/c5e355d03452/foods-13-02384-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/07ceb3566b65/foods-13-02384-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/06d5d3d3ac58/foods-13-02384-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/39aa30d614ab/foods-13-02384-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/b83f5785009d/foods-13-02384-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/83d2766a630c/foods-13-02384-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a6/11311554/c5e355d03452/foods-13-02384-g006.jpg

相似文献

[1]
Investigation into Antioxidant Mechanism of Extract in Carbendazim-Induced PC12 Cell Injury Model through Transcriptomics and Metabolomics Analyses.

Foods. 2024-7-28

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[6]
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[7]
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[8]
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[9]
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[10]
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本文引用的文献

[1]
Novel antioxidant peptides identified from coix seed by molecular docking, quantum chemical calculations and invitro study in HepG2 cells.

Food Chem. 2024-5-15

[2]
Reactive Oxygen Species Damage Bovine Endometrial Epithelial Cells via the Cytochrome C-mPTP Pathway.

Antioxidants (Basel). 2023-12-16

[3]
The ubiquitin conjugase Rad6 mediates ribosome pausing during oxidative stress.

Cell Rep. 2023-11-28

[4]
Sijunzi decoction ameliorates gastric precancerous lesions via regulating oxidative phosphorylation based on proteomics and metabolomics.

J Ethnopharmacol. 2024-1-10

[5]
Integrated transcriptome and metabolome provide insight into flavonoid variation in goji berries (Lycium barbarum L.) from different areas in China.

Plant Physiol Biochem. 2023-6

[6]
Methods and applications for single-cell and spatial multi-omics.

Nat Rev Genet. 2023-8

[7]
Deciphering mechanism of Zhishi-Xiebai-Guizhi Decoction against hypoxia/reoxygenation injury in cardiomyocytes by cell metabolomics: Regulation of oxidative stress and energy acquisition.

J Chromatogr B Analyt Technol Biomed Life Sci. 2023-2-1

[8]
Metabolomics study based on GC-MS reveals a protective function of luteolin against glutamate-induced PC12 cell injury.

Biomed Chromatogr. 2023-2

[9]
Investigation into the anti-inflammatory mechanism of coffee leaf extract in LPS-induced Caco-2/U937 co-culture model through cytokines and NMR-based untargeted metabolomics analyses.

Food Chem. 2023-3-15

[10]
Mitochondrial ROS-mediated ribosome stalling and GCN2 activation are partially involved in 1-nitropyrene-induced steroidogenic inhibition in testes.

Environ Int. 2022-9

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