Yan Huifang, Tian Daicai, Zhang Qian, Wen Jiangqi, Wang Zeng-Yu, Chai Maofeng
Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China.
Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK 73401, USA.
Plants (Basel). 2024 Jul 26;13(15):2061. doi: 10.3390/plants13152061.
Seed physical dormancy (hard-seededness) is an interesting ecological phenomenon and important agronomic trait. The loss of seed coat impermeability/hard-seededness is a key target trait during the domestication of leguminous crops which allows seeds to germinate rapidly and uniformly. In this study, we examined the mutation of quantitative trait locus (QTL) genes, and , in 18 wild soybean () and 23 cultivated soybean () accessions. The sequencing results indicate that a G-to-T substitution in and a C-to-T substitution in occurred in all 23 cultivated soybean accessions but not in any of the 18 wild soybean accessions. The mutations in the two genes led to increased seed coat permeability in cultivated soybean. Therefore, we provide evidence that two genes, and , simultaneously contribute to the domestication of hard-seededness in soybeans. This finding is of great significance for genetic analysis and improved utilization of the soybean hard-seededness trait.
种子物理休眠（硬实性）是一种有趣的生态现象和重要的农艺性状。种皮不透性/硬实性的丧失是豆科作物驯化过程中的关键目标性状，它能使种子快速、均匀地萌发。在本研究中，我们检测了18份野生大豆（Glycine soja）和23份栽培大豆（Glycine max）种质中数量性状位点（QTL）基因 和 的突变情况。测序结果表明，所有23份栽培大豆种质中均发生了 基因的G到T替换以及 基因的C到T替换，而18份野生大豆种质中均未出现这些替换。这两个基因的突变导致栽培大豆种皮通透性增加。因此，我们提供了证据表明 基因和 基因同时促成了大豆硬实性的驯化。这一发现对于大豆硬实性性状的遗传分析和改良利用具有重要意义。
