Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, 1111 Budapest, Hungary.
CycloLab Cyclodextrin R & D Laboratory Ltd., 1097 Budapest, Hungary.
Molecules. 2024 Aug 5;29(15):3698. doi: 10.3390/molecules29153698.
Cyclodextrins, commonly used as excipients in antifungal formulations to improve the physicochemical properties and availability of the host molecules, have not been systematically studied for their effects and bioactivity without a complex active substance. This paper evaluates the effects of various cyclodextrins on the physiology of the test organism . The research examines their impact on yeast growth, viability, biofilm formation and morphological changes. Native ACD, BCD, randomly methylated α- and β-CD and quaternary ammonium α-CD and β-CD were investigated in the 0.5-12.5 mM concentration range in both static and dynamic systems. The study revealed that certain cyclodextrins exhibited notable antifungal effects (up to ~69%) in dynamic systems; however, the biofilm formation was enhanced in static systems. The magnitude of these effects was influenced by several variables, including the size of the internal cavity, the concentration and structure of the cyclodextrins, and the contact time. Furthermore, the study found that CDs exhibited distinct effects in both static and dynamic systems, potentially related to their tendency to form aggregates. The findings suggest that cyclodextrins may have the potential to act as antifungal agents or growth promoters, depending on their structure and surrounding environments.
环糊精通常被用作抗真菌制剂中的赋形剂，以改善宿主分子的物理化学性质和可用性，但在没有复杂活性物质的情况下，它们对其影响和生物活性尚未进行系统研究。本文评估了各种环糊精对试验生物的生理学的影响。该研究考察了它们对酵母生长、活力、生物膜形成和形态变化的影响。在静态和动态系统中，研究了 0.5-12.5 mM 浓度范围内的天然 ACD、BCD、随机甲基化的α-和β-CD 以及季铵化的α-CD 和β-CD。研究表明，某些环糊精在动态系统中表现出显著的抗真菌作用（高达约 69%）；然而，在静态系统中生物膜形成得到增强。这些作用的大小受到几个变量的影响，包括内部空腔的大小、环糊精的浓度和结构以及接触时间。此外，研究发现，CD 在静态和动态系统中都表现出明显的作用，这可能与其形成聚集体的倾向有关。研究结果表明，环糊精可能具有作为抗真菌剂或生长促进剂的潜力，这取决于它们的结构和周围环境。
