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通过比较冷藏过程中 和 ZPX 竹笋的木质化分析鉴定关键基因。

Identification of the Key Gene through Comparative Analysis of Lignification in and ZPX Bamboo Shoots during Cold Storage.

机构信息

Laboratory of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang 621010, China.

Sichuan Provincial Forestry and Grass Land Key Laboratory for Conservation and Sustainable Utilization of Bamboo Genetic Resources in Southwest of China, Mianyang 621010, China.

出版信息

Int J Mol Sci. 2024 Jul 24;25(15):8065. doi: 10.3390/ijms25158065.

Abstract

bamboo shoots, a species with rich nutritional value, are important in Southwest China. Lignin is an important factor affecting the postharvest flavor quality of bamboo shoots; however, the underlying mechanism of lignin deposition in bamboo shoots during cold storage is still not fully understood. In this study, the mutant with low lignin content at 3.11% and the cultivated variety ZPX at 4.47% were used as experimental materials. The lignin content of and ZPX, as well as the gene expression differences between them, were compared and analyzed during cold storage using transcriptomic and physiological methods. Our analysis revealed several key genes and found that plays a key role in the regulatory network of bamboo shoots during cold storage. Tobacco heterologous transformation experiments demonstrated that overexpression of significantly increases lignin content. This study provides a novel foundation for future research aimed at improving the postharvest quality and flavor of bamboo shoots through targeted genetic manipulation during cold storage.

摘要

竹笋是一种具有丰富营养价值的物种,在中国西南部非常重要。木质素是影响竹笋采后风味品质的重要因素,但木质素在竹笋冷藏过程中的沉积机制仍不完全清楚。在这项研究中,我们使用木质素含量分别为 3.11%的突变体和 4.47%的栽培品种 ZPX 作为实验材料。通过转录组学和生理方法比较和分析了它们在冷藏过程中的木质素含量以及它们之间的基因表达差异。我们的分析揭示了几个关键基因,并发现 在竹笋冷藏过程中的调控网络中起着关键作用。烟草异源转化实验表明, 的过表达显著增加了木质素含量。这项研究为未来通过在冷藏过程中的靶向遗传操作来改善竹笋的采后品质和风味提供了新的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b36/11311333/1798f6f32807/ijms-25-08065-g001.jpg

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