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一个甘薯 R2R3-MYB 基因，提高了转基因烟草的耐盐性。

, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco.

机构信息

College of Agriculture, Yangtze University, Jingzhou 434025, China.

Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430072, China.

出版信息

Genes (Basel). 2022 Aug 18;13(8):1476. doi: 10.3390/genes13081476.

DOI:10.3390/genes13081476
PMID:36011387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9408268/
Abstract

The MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor family plays an important role in plant growth, development, and response to biotic and abiotic stresses. However, the gene functions of MYB transcription factors in sweet potato ( (L.) Lam) have not been elucidated. In this study, an MYB transcription factor gene, , was identified and isolated from sweet potato. Multiple sequence alignment showed that IbMYB308 is a typical R2R3-MYB transcription factor. Further, quantitative real-time PCR (qRT-PCR) analysis revealed that was expressed in root, stem, and, especially, leaf tissues. Moreover, it showed that had a tissue-specific profile. The experiment also showed that the expression of was induced by different abiotic stresses (20% PEG-6000, 200 mM NaCl, and 20% HO). After a 200 mM NaCl treatment, the expression of several stress-related genes (, , , and ) was upregulation in transgenic plants, and the CAT activity, POD activity, proline content, and protein content in transgenic tobacco had increased, while MDA content had decreased. In conclusion, this study demonstrated that could improve salt stress tolerance in transgenic tobacco. These findings lay a foundation for future studies on the R2R3-MYB gene family of sweet potato and suggest that could potentially be used as an important positive factor in transgenic plant breeding to improve salt stress tolerance in sweet potato plants.

摘要

MYB（v-myb 禽成髓细胞瘤病毒癌基因同源物）转录因子家族在植物的生长、发育以及对生物和非生物胁迫的响应中起着重要作用。然而，MYB 转录因子在甘薯中的基因功能尚未阐明。在本研究中，从甘薯中鉴定并分离出一个 MYB 转录因子基因 IbMYB308。多重序列比对表明 IbMYB308 是一种典型的 R2R3-MYB 转录因子。进一步的定量实时 PCR（qRT-PCR）分析表明，IbMYB308 在根、茎和叶组织中表达，尤其是在叶组织中表达量较高。此外，它表现出组织特异性的表达模式。实验还表明，IbMYB308 的表达受不同非生物胁迫（20%PEG-6000、200mM NaCl 和 20%HO）的诱导。在 200mM NaCl 处理后，转基因植株中几种与胁迫相关基因（、、、和）的表达上调，而转基因烟草中的 CAT 活性、POD 活性、脯氨酸含量和蛋白质含量增加，MDA 含量降低。综上所述，本研究表明 IbMYB308 可以提高转基因烟草的耐盐性。这些发现为甘薯 R2R3-MYB 基因家族的未来研究奠定了基础，并表明 IbMYB308 可能作为转基因植物育种中的一个重要正因子，用于提高甘薯植株的耐盐性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/383086873e52/genes-13-01476-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/052d8dd82e16/genes-13-01476-g001a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/9f90851779d5/genes-13-01476-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/7b9aea1ad132/genes-13-01476-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/4163ee6b986b/genes-13-01476-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/55bca86c95dc/genes-13-01476-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/8814eff6ff97/genes-13-01476-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/383086873e52/genes-13-01476-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/052d8dd82e16/genes-13-01476-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/8fb66bbc8272/genes-13-01476-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/9f90851779d5/genes-13-01476-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/7b9aea1ad132/genes-13-01476-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/4163ee6b986b/genes-13-01476-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/55bca86c95dc/genes-13-01476-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/8814eff6ff97/genes-13-01476-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/9408268/383086873e52/genes-13-01476-g008.jpg

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3
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6
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5
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