Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, 999078, China.
Macau University of Science and Technology, Macau, 999078, China.
J Orthop Surg Res. 2024 Aug 10;19(1):474. doi: 10.1186/s13018-024-04826-5.
Anterior talofibular ligament injuries and osteochondral lesions of the talus present unique challenges to orthopedic surgeons. This study aimed to investigate the relevant relationship between them by analyzing the Magnetic resonance imaging (MRI) results of clinical patients and single-cell RNA sequence (scRNA seq) results of healthy talus cartilage to discuss the risk factors. Data from 164 patients from 2018 to 2023 was retrospectively analyzed. The correlation analysis between ATFL injury grade and the Hepple stage of OLT determined by MRI was performed. Publicly available single-cell RNA datasets were collected. Single-cell RNA datasets from five volunteers of healthy talus cartilage were analyzed. ATFL injury grade was relevant with the Hepple stage of OLT (P < 0.05). The results of multivariate logistic regression analysis showed that injured area was the independent influencing factor of the incidence rate and the severity of OLT (P < 0.05). The Hepple stage of OLT was relevant with AOFAS and VAS (P < 0.05). Single-cell RNA sequence results showed that among the 9 subtypes of chondrocytes, the interaction strength between HTC-A and HTC-B is the highest. Their physical interactions are mainly achieved through the CD99 signaling pathway, and factor interactions are mainly achieved through the ANGPTL signaling pathway. Anterior talofibular ligament injury may lead to osteochondral lesions of the talus. Early medical intervention should be carried out for ligament injuries to restore joint stability and avoid cartilage damage.
距腓前韧带损伤和距骨骨软骨病变给骨科医生带来了独特的挑战。本研究旨在通过分析临床患者的磁共振成像（MRI）结果和健康距骨软骨的单细胞 RNA 序列（scRNA seq）结果，探讨相关关系，并讨论其危险因素。回顾性分析了 2018 年至 2023 年的 164 例患者的数据。对 MRI 确定的 ATFL 损伤分级与 OLT 的 Hepple 分期之间的相关性进行了分析。收集了公开的单细胞 RNA 数据集。对来自五个健康距骨软骨志愿者的单细胞 RNA 数据集进行了分析。ATFL 损伤分级与 OLT 的 Hepple 分期相关（P<0.05）。多因素 logistic 回归分析结果表明，损伤面积是 OLT 发生率和严重程度的独立影响因素（P<0.05）。OLT 的 Hepple 分期与 AOFAS 和 VAS 相关（P<0.05）。单细胞 RNA 序列结果显示，在 9 种软骨细胞亚型中，HTC-A 和 HTC-B 之间的相互作用强度最高。它们的物理相互作用主要通过 CD99 信号通路实现，而因子相互作用主要通过 ANGPTL 信号通路实现。距腓前韧带损伤可能导致距骨骨软骨病变。对于韧带损伤，应尽早进行医学干预，以恢复关节稳定性，避免软骨损伤。
