College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China.
College of Agriculture and Bioengineering, Heze University, Heze 274000, China.
Molecules. 2023 Jul 14;28(14):5405. doi: 10.3390/molecules28145405.
Antibiotic resistance to pathogenic bacteria is becoming an increasing public health threat, and identifying alternatives to antibiotics would be an effective solution to the problem of drug resistance. Antimicrobial peptides are small peptides produced by various organisms; they are considered to be adequate antibiotic substitutes because they have intense, broad-spectrum antibacterial activity and stability, are widely available, and target strains do not quickly develop resistance. Recent research on antimicrobial peptides has shown that they have broad potential for applications in medicine, agriculture, food, and animal feed. Turgencin A is a potent antimicrobial peptide isolated from the Arctic sea squirt. We established a His-tagged expression system for and developed a rTurgencin A using the recombinant expression in with nickel column purification. This antimicrobial peptide showed intense antimicrobial activity against Gram-positive and Gram-negative bacteria and a good stability at most temperatures and pHs, as well as in various protease and salt ion concentrations, but underwent a significant decrease in stability in high-temperature and low-pH environments. Turgencin A induced bacterial membrane rupture, resulting in content leakage and subsequent cell death. It was also shown to have low hemolytic activity. This study provides primary data for the industrial production and application of the antimicrobial peptide Turgencin A.
抗生素耐药性对致病菌而言是日益严重的公共卫生威胁，而寻找抗生素替代品将是解决耐药性问题的有效方法。抗菌肽是由各种生物体产生的小肽；它们被认为是合适的抗生素替代品，因为它们具有强烈、广谱的抗菌活性和稳定性，来源广泛，且靶菌株不会迅速产生耐药性。抗菌肽的最新研究表明，它们在医学、农业、食品和动物饲料等领域具有广泛的应用潜力。Turgencin A 是一种从北极海鞘中分离出的强效抗菌肽。我们建立了 的 His 标签表达系统，并利用重组表达在 中开发了 rTurgencin A，采用镍柱纯化。这种抗菌肽对革兰氏阳性菌和革兰氏阴性菌具有强烈的抗菌活性，在大多数温度和 pH 值、各种蛋白酶和盐离子浓度下具有良好的稳定性，但在高温和低 pH 环境下稳定性显著下降。Turgencin A 诱导细菌膜破裂，导致内容物泄漏和随后的细胞死亡。它的溶血活性也较低。本研究为抗菌肽 Turgencin A 的工业生产和应用提供了初步数据。
