Araujo Natália Gonçalves Ribeiro, Araujo-Lima Carlos Fernando, Oliveira Renatha Tavares de, Macedo Andrea Furtado, Felzenszwalb Israel
Laboratory of Environmental Mutagenesis (LABMUT), University of Rio de Janeiro State (UERJ), Rio de Janeiro, RJ, Brazil.
Laboratory of Pharmaceutical and Technological Innovation, Federal University of Rio de Janeiro State (UNIRIO), Rio de Janeiro, RJ, Brazil.
Toxicol Rep. 2024 Jul 14;13:101693. doi: 10.1016/j.toxrep.2024.101693. eCollection 2024 Dec.
The genus is crucial for global production in food, perfume, and pharmaceutical industries. However, exploitation threatens some species, leading to extinction. Traditional communities use vanilla for medicinal purposes, and there are species like Klotzsch and Hoehne with potential to occupy the market. For this, methanolic extraction of these two mentioned species was conducted alongside . Analyzes of the cell viability, mutagenic and genotoxic potential were performed. In the Ames test, the assays were performed with concentrations from 0.5 and 5000 μg/ml and on five strains. Only exhibited mutagenicity at the highest concentration in the TA98 strain. Viability tests were performed within a dose range of 0.05-5000 µg/ml and 24, 48, and 72-hour exposures. It was possible to observe a reduction in cell viability observed only at the highest concentration, for all three species and both cell types tested. Genotoxicity induction by the extracts was assessed at concentrations from 0.5 to 500 µg/ml through the cytokinesis-block micronucleus assay. No genotoxic damage or reduction in the Nucleus Division Index (NDI). The study found no mutagenicity, cytotoxicity, or genotoxicity in the species tested, indicating potential human use for food or pharmaceutical purposes.
该属对于食品、香水和制药行业的全球生产至关重要。然而，过度开发正威胁着一些物种，导致其灭绝。传统社区将香草用于药用，还有如克洛茨希香草和霍氏香草等有潜力占领市场的物种。为此，对上述这两个物种进行了甲醇提取，并开展了细胞活力、诱变和遗传毒性潜力分析。在艾姆斯试验中，检测浓度为0.5至5000μg/ml，共对五种菌株进行了检测。仅在TA98菌株中，最高浓度时表现出致突变性。活力测试在0.05 - 5000µg/ml的剂量范围内进行，暴露时间为24、48和72小时。对于所有三个物种和两种受试细胞类型，仅在最高浓度时观察到细胞活力下降。通过胞质分裂阻滞微核试验，在0.5至500μg/ml的浓度下评估提取物的遗传毒性诱导情况。未发现遗传毒性损伤或核分裂指数（NDI）降低。该研究在受试物种中未发现致突变性、细胞毒性或遗传毒性，表明这些物种有用于食品或制药目的的潜在价值。
