Gbégbé Dého Aristide, Kacou Okran Beyosse Christophe, N'zi N'goran Parfait, Angaman Djédoux Maxime
Department of Biochemistry-Microbiology Jean Lorougnon Guédé University, Daloa, Côte d'Ivoire.
Department of Bacteriology-Virology National Reference Center for Antibiotics Institut Pasteur, Abidjan, Côte d'Ivoire.
Int J Genomics. 2024 Aug 3;2024:6239250. doi: 10.1155/2024/6239250. eCollection 2024.
Bacterial strains coded 21LM367, 21LM07, and 21LM1136 were isolated from the urine of patients with urinary tract infections (UTIs) at the Centre Hospitalier Régional de Daloa in Côte d'Ivoire. Based on average nucleotide identity (ANI) analysis, DNA-DNA digital hybridisation (dDDH), and other comparative genomic methods, strains 21LM07, 21LM367, and 21LM1136 were determined to be . The size of the assembled complete genomes ranged from 8,624,538 to 4,007,501 bp. The average GC content was 37.76%, 46.33%, and 43.03% for strains 21LM07, 21LM367, and 21LM1136, respectively. The total number of coding regions (CDS) in each genome was 4172, 8497, and 6795, respectively, for strains 21LM07, 21LM367, and 21LM1136. Genomic prediction analysis revealed that a total of 4241, 8583, and 6881 genes were annotated in the 21LM07, 21LM367, and 21LM1136 genomes, respectively. No virulence or resistance genes were predicted in the genomes of strains 21LM07 and 21LM1136. On the other hand, two genes conferring resistance to beta-lactam and tetracyclines as well as nine virulence genes were predicted in the genome of 21LM367. In addition, 438, 350, and 153 mobile genetic elements (MGEs) were predicted in the genomes of strains 21LM367, 21LM1136, and 21LM07, respectively. Strain 21LM07 was characterised by the absence of plasmids in its genome. Two plasmids were predicted in the genomes of isolates 21LM367 and 21LM1136; however, rep7a and IncI2 were predicted to contain the tet(K) resistance gene. No typical multilocus sequences could be characterised in the genomes of the different strains.
编码为21LM367、21LM07和21LM1136的细菌菌株，是从科特迪瓦洛阿地区中心医院尿路感染（UTI）患者的尿液中分离出来的。基于平均核苷酸同一性（ANI）分析、DNA-DNA数字杂交（dDDH）和其他比较基因组方法，确定21LM07、21LM367和21LM1136菌株为[此处原文缺失相关信息]。组装完成的基因组大小范围为8,624,538至4,007,501 bp。21LM07、21LM367和21LM1136菌株的平均GC含量分别为37.76%、46.33%和43.03%。21LM07、21LM367和21LM1136菌株每个基因组中的编码区（CDS）总数分别为4172、8497和6795。基因组预测分析显示，21LM07、21LM367和21LM1136基因组中分别注释了总共4241、8583和6881个基因。在21LM07和21LM1136菌株的基因组中未预测到毒力或抗性基因。另一方面，在21LM367的基因组中预测到两个赋予对β-内酰胺和四环素抗性的基因以及九个毒力基因。此外，在21LM367、21LM1136和21LM07菌株的基因组中分别预测到438、350和153个移动遗传元件（MGE）。21LM07菌株的特征是其基因组中没有质粒。在21LM367和21LM1136分离株的基因组中预测到两个质粒；然而，rep7a和IncI2预计含有tet(K)抗性基因。在不同菌株的基因组中无法鉴定出典型的多位点序列。
