Ortega-Urquieta Maria Edith, Valenzuela-Ruíz Valeria, Mitra Debasis, Hyder Sajjad, Elsheery Nabil I, Kumar Das Mohapatra Pradeep, Parra-Cota Fannie Isela, de Los Santos-Villalobos Sergio
Instituto Tecnológico de Sonora, 5 de Febrero 818 sur, Ciudad Obregon 85000, Sonora, Mexico.
Department of Microbiology, Raiganj University, Raiganj 733 134, Uttar Dinajpur, West Bengal India, India.
Plants (Basel). 2022 Aug 28;11(17):2231. doi: 10.3390/plants11172231.
Strain TSO9 was isolated from a commercial field of wheat ( L. subsp. ) located in the Yaqui, Valley, Mexico. Here, the genome of this strain was sequenced, obtaining a total of 5,248,515 bp; 38.0% G + C content; 1,186,514 bp N50; and 2 L50. Based on the 16S rRNA gene sequencing, strain TSO9 was affiliated with the genus . The genome annotation of sp. TSO9 contains a total of 147 RNAs, 128 tRNAs, 1 tmRNA, and 5512 coding DNA sequences (CDS) distributed into 332 subsystems, where CDS associated with agricultural purposes were identified, such as (i) virulence, disease, and defense (57 CDS) (i.e., resistance to antibiotics and toxic compounds (34 CDS), invasion and intracellular resistance (12 CDS), and bacteriocins and ribosomally synthesized antibacterial peptides (10 CDS)), (ii) iron acquisition and metabolism (36 CDS), and (iii) secondary metabolism (4 CDS), i.e., auxin biosynthesis. In addition, subsystems related to the viability of an active ingredient for agricultural bioproducts were identified, such as (i) stress response (65 CDS). These genomic traits are correlated with the metabolic background of this strain, and its positive effects on wheat growth regulation reported in this work. Thus, further investigations of sp. TSO9 are necessary to complement findings regarding its application in agroecosystems to increase wheat yield sustainably.
菌株TSO9是从墨西哥亚基河谷一个商业化种植的小麦（L.亚种）田中分离得到的。在此，对该菌株的基因组进行了测序，共获得5,248,515 bp；G + C含量为38.0%；N50为1,186,514 bp；L50为2。基于16S rRNA基因测序，菌株TSO9隶属于 属。TSO9 种的基因组注释共包含147个RNA、128个tRNA、1个tmRNA和5512个编码DNA序列（CDS），这些序列分布在332个子系统中，其中鉴定出了与农业目的相关的CDS，例如：（i）毒力、疾病和防御（57个CDS）（即对抗生素和有毒化合物的抗性（34个CDS）、侵袭和细胞内抗性（12个CDS）以及细菌素和核糖体合成的抗菌肽（10个CDS）），（ii）铁的获取与代谢（36个CDS），以及（iii）次级代谢（4个CDS），即生长素生物合成。此外，还鉴定出了与农业生物产品活性成分活力相关的子系统，例如：（i）应激反应（65个CDS）。这些基因组特征与该菌株的代谢背景及其在本研究中报道的对小麦生长调节的积极作用相关。因此，有必要对TSO9 种进行进一步研究，以补充其在农业生态系统中应用以可持续提高小麦产量方面的研究结果。
