Marine College, Shandong University, Weihai, Shandong, 264209, PR China.
Joint Science College, Shandong University, Weihai, Shandong, 264209, PR China.
Int J Syst Evol Microbiol. 2024 Aug;74(8). doi: 10.1099/ijsem.0.006490.
Two novel rod-shaped, strictly aerobic, non-motile and Gram-stain-negative bacterial strains, designated SDUM040013 and SDUM040014, were isolated from kelp seedlings in Weihai, PR China. Cells of strain SDUM040013 were 0.3-0.4 µm wide and 0.8-1.8 µm long, catalase-positive and oxidase-positive. Growth of SDUM040013 was observed at 0-37 °C (optimum, 28-30 °C) and pH 5.5-9 (optimum, pH 8.0) and in the presence of 1-8 % (w/v) NaCl (optimum, 2 %). The DNA G+C content of strain SDUM040013 was 50.5 %. Strain SDUM040013 showed the highest 16S rRNA gene sequence similarity (97.1 %) to . Cells of strain SDUM040014 were 0.4-0.5 µm wide and 1.0-1.4 µm long, catalase-positive and oxidase-positive. Growth of SDUM040014 was observed at 4-40 °C (optimum, 28-30 °C) and pH 5.5-9 (optimum, pH 8.5) and in the presence of 0-8 % (w/v) NaCl (optimum, 2 %). The DNA G+C content of strain SDUM040014 was 56.5 %. Strain SDUM040014 showed the highest 16S rRNA gene sequence similarity (96.2%) to . The isoprenoid quinone of both strains was Q-8 and the predominant fatty acids were summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7) and C. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. Given these phenotypic and chemotaxonomic properties, as well as phylogenetic data, strains SDUM040013 and SDUM040014 were considered to represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains are SDUM040013 (=KCTC 8123=MCCC 1H01413) and SDUM040014 (=KCTC 8124=MCCC 1H01414), respectively.
两株新型杆状、严格需氧、不运动、革兰氏阴性细菌菌株，分别命名为 SDUM040013 和 SDUM040014，从中国威海的海带幼苗中分离得到。菌株 SDUM040013 的细胞宽 0.3-0.4μm，长 0.8-1.8μm，过氧化氢酶阳性，氧化酶阳性。SDUM040013 的生长温度为 0-37°C（最佳温度为 28-30°C），pH 值为 5.5-9（最佳 pH 值为 8.0），并可在 1-8%（w/v）NaCl（最佳浓度为 2%）存在的情况下生长。菌株 SDUM040013 的 DNA G+C 含量为 50.5%。菌株 SDUM040013 与. 的 16S rRNA 基因序列相似度最高（97.1%）。菌株 SDUM040014 的细胞宽 0.4-0.5μm，长 1.0-1.4μm，过氧化氢酶阳性，氧化酶阳性。SDUM040014 的生长温度为 4-40°C（最佳温度为 28-30°C），pH 值为 5.5-9（最佳 pH 值为 8.5），并可在 0-8%（w/v）NaCl（最佳浓度为 2%）存在的情况下生长。菌株 SDUM040014 的 DNA G+C 含量为 56.5%。菌株 SDUM040014 与. 的 16S rRNA 基因序列相似度最高（96.2%）。两株菌的类异戊二烯醌均为 Q-8，主要脂肪酸为 sum feature 3（C 7 和/或 C 6）、sum feature 8（C 7）和 C。二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺是主要的极性脂。基于这些表型和化学分类学特性以及系统发育数据，菌株 SDUM040013 和 SDUM040014 被认为代表了. 属的两个新种，分别命名为. sp. nov. 和. sp. nov.。模式菌株分别为 SDUM040013（=KCTC 8123=MCCC 1H01413）和 SDUM040014（=KCTC 8124=MCCC 1H01414）。
