两新种，从潮滩沉积物中分离得到的两个新型物种。

sp. nov. and sp. nov., two novel species isolated from tidal flat sediments.

机构信息

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.

Shaoxing Biomedical Research Institute of Zhejiang Sci-Tech University Co., Ltd., Zhejiang Engineering Research Center for the Development Technology of Medicinal and Edible Homologous Health Food, Shaoxing 312075, PR China.

出版信息

Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006469.

DOI:10.1099/ijsem.0.006469

PMID:39052323

Abstract

Two Gram-stain-negative, rod-shaped, non-motile, aerobic and carotenoid-producing strains, belonging to the family designated as H149 and Z2, were isolated from tidal flat sediment samples collected in Hainan and Zhejiang, PR China, respectively. Growth of strain H149 occurred at 15-42 °C, 0-10.0 % (w/v) NaCl, and pH 6.0-8.5, with the optima at 35-37 °C, 3.0-3.5 % (w/v) NaCl and pH 7.0. Strain Z2 grew at 15-37 °C, 0-6.0 % (w/v) NaCl, and pH 6.0-9.5, with the optima at 25-30 °C, 0.5-1.0 % (w/v) NaCl and pH 6.0-6.5. Ubiquinone-10 was the sole ubiquinone in two strains. The predominant cellular fatty acids of strain H149 were C, summed feature 3 and summed feature 8, while those of strain Z2 were C 6, summed feature 3 and summed feature 8. Strains H149 and Z2 shared diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid as major polar lipids. The 16S rRNA gene sequence identity analysis indicated that strain H149 had the highest sequence identity of 98.4 % with KCTC 23981, and strain Z2 had that of 98.2 % with NZ-96. Phylogenetic trees based on 16S rRNA gene and core-genome sequences revealed that strains H149 and Z2 formed two independent clades in the genera and , respectively. Strain H149 had average nucleotide identity values of 74.0-81.3 % and DNA-DNA hybridization values of 18.5-23.1 % with type strains, while strain Z2 had values of 73.3-78.7 % and 14.5-33.3 % with type strains. The genomic DNA G+C contents of strains H149 and Z2 were 64.3 and 61.8 %, respectively. Based on the genetic, genomic, phylogenetic, physiological and chemotaxonomic results, strains H149 (=KCTC 8397=MCCC 1K08920) and Z2 (=KCTC 8396=MCCC 1K08946) are concluded to represent two novel species for which the names sp. nov. and sp. nov. are proposed, respectively.

摘要

两株革兰氏阴性、杆状、非运动、需氧和类胡萝卜素产生的菌株，分别属于 H149 和 Z2 两个家族，从中国海南和浙江的潮间带沉积物样本中分离得到。菌株 H149 的生长温度为 15-42°C，盐度为 0-10.0%（w/v），pH 值为 6.0-8.5，最适生长温度为 35-37°C，盐度为 3.0-3.5%（w/v），pH 值为 7.0。菌株 Z2 的生长温度为 15-37°C，盐度为 0-6.0%（w/v），pH 值为 6.0-9.5，最适生长温度为 25-30°C，盐度为 0.5-1.0%（w/v），pH 值为 6.0-6.5。两株菌均含有醌-10 作为唯一的类异戊二烯醌。菌株 H149 的主要细胞脂肪酸为 C 、特征 3 和特征 8，而菌株 Z2 的主要细胞脂肪酸为 C 6 、特征 3 和特征 8。菌株 H149 和 Z2 共有的极性脂有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和神经鞘糖脂。16S rRNA 基因序列分析表明，菌株 H149 与 KCTC 23981 的序列相似度最高，为 98.4%，菌株 Z2 与 NZ-96 的序列相似度最高，为 98.2%。基于 16S rRNA 基因和核心基因组序列构建的系统发育树表明，菌株 H149 和 Z2 分别在属和属中形成两个独立的分支。菌株 H149 与模式菌株的平均核苷酸同一性值为 74.0-81.3%，DNA-DNA 杂交值为 18.5-23.1%，而菌株 Z2 与模式菌株的平均核苷酸同一性值为 73.3-78.7%，DNA-DNA 杂交值为 14.5-33.3%。菌株 H149 和 Z2 的基因组 DNA G+C 含量分别为 64.3%和 61.8%。基于遗传、基因组、系统发育、生理和化学分类学的结果，菌株 H149（=KCTC 8397=MCCC 1K08920）和 Z2（=KCTC 8396=MCCC 1K08946）被鉴定为两个新的属，分别命名为 sp. nov. 和 sp. nov.。