大肠杆菌热稳定肠毒素的制备性纯化
Preparative purification of Escherichia coli heat-stable enterotoxin.
作者信息
Saeed A M, Greenberg R N
出版信息
Anal Biochem. 1985 Dec;151(2):431-7. doi: 10.1016/0003-2697(85)90200-3.
Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures.
从牛源大肠杆菌菌株中分离出的热稳定肠毒素（STa），通过在化学成分明确的培养基中培养该细菌菌株、脱盐，并采用Amberlite XAD - 2树脂进行分批吸附色谱法、反相硅胶分批吸附色谱法以及制备型反相高效液相色谱法对培养滤液进行浓缩，从而纯化至同质状态。这种快速的制备性纯化方案获得了高回收率的纯STa，其与通过更复杂程序纯化的STa表现出生化同源性。
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