产肠毒素大肠杆菌牛和猪菌株产生的两种不同热稳定肠毒素的分离及部分特性鉴定
Isolation and partial characterization of two different heat-stable enterotoxins produced by bovine and porcine strains of enterotoxigenic Escherichia coli.
作者信息
Kapitany R A, Forsyth G W, Scoot A, McKenzie S F, Worthington R W
出版信息
Infect Immun. 1979 Oct;26(1):173-7. doi: 10.1128/iai.26.1.173-177.1979.
Abstract
Heat-stable enterotoxins (ST-124 and ST-1261) have been isolated from two different enterotoxigenic Escherichia coli of bovine (124) and porcine (1261) origin. The enterotoxin preparations were isolated by ultrafiltration and ion-exchange chromatography and were both active in the suckling mouse test and pig ligated loop test in the nanogram range. The bovine (ST-124) enterotoxin was not stable to heating in its isolated form, and significant differences in amino acid composition were observed between the two enterotoxins. Although both toxins were active at similar levels in the suckling mouse and pig ligated loop tests, ST-124 lacked the ability to cause the profound secretory responses seen with ST-1261 in the weanling pig ligated loop.
摘要
已从两种不同来源（牛源的124型和猪源的1261型）的产肠毒素大肠杆菌中分离出热稳定肠毒素（ST - 124和ST - 1261）。肠毒素制剂通过超滤和离子交换色谱法分离得到，在乳鼠试验和猪肠结扎环试验中，二者在纳克范围内均具有活性。牛源（ST - 124）肠毒素以分离形式存在时对加热不稳定，且两种肠毒素在氨基酸组成上存在显著差异。尽管两种毒素在乳鼠试验和猪肠结扎环试验中的活性水平相似，但ST - 124缺乏在断奶仔猪肠结扎环试验中引发如ST - 1261所见的深度分泌反应的能力。
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