Bischoff R, McLaughlin L W
Anal Biochem. 1985 Dec;151(2):526-33. doi: 10.1016/0003-2697(85)90215-5.
The coating of a C18-reversed-phase high performance liquid chromatography support (octadecylsilyl-Hypersil) with a tetraalkylammonium salt (methyltrioctylammonium chloride) produces a chromatographic matrix with both ionic and hydrophobic character. Using this material oligonucleotides and tRNAs can be separated with high resolution. The observed resolution is in part due to the apparent lack of diffusion processes occurring during chromatography with this matrix. Some tRNAs can be obtained in high purity from a bulk tRNA mixture after a single chromatographic step. In general it is more efficient to use the matrix as the last step of a purification procedure for a particular tRNA. A two-step procedure is described which allows, in some cases, the isolation of small quantities of specific tRNA isoacceptors.
用四烷基铵盐（甲基三辛基氯化铵）包覆C18反相高效液相色谱载体（十八烷基硅烷化高效硅胶）可制得具有离子和疏水特性的色谱基质。使用这种材料可以高分辨率分离寡核苷酸和tRNA。观察到的分辨率部分归因于在此基质上进行色谱分析时明显缺乏扩散过程。经过单步色谱分析后，一些tRNA可从大量tRNA混合物中获得高纯度产品。一般来说，将该基质用作特定tRNA纯化程序的最后一步更为有效。本文描述了一种两步法，在某些情况下，该方法可用于分离少量特定的tRNA同功受体。
