Bischoff R, Graeser E, McLaughlin L W
J Chromatogr. 1983 Mar 4;257(2):305-15. doi: 10.1016/s0021-9673(01)88186-3.
High-performance liquid chromatography on a reversed-phase support treated with a tetraalkylammonium salt was used to separate tRNAs from baker's yeast. While resolution by this column appears to result from both anion-exchange and reversed-phase chromatography, it is the hydrophobic interactions which govern the separation of one tRNA from another. Chromatography of bulk tRNA resulted in a number of fractions with different amino acid acceptor activities and little cross-contamination. In some cases the column resolved several single nucleotide modifications of tRNAPhe. Using a 250 x 6.2 mm column it has been possible to chromatograph a minimum of 100 A260 units of tRNA without serious loss in resolution. tRNAs isolated from this column as the last step of a purification procedure have very high amino acid acceptor activities.
使用经四烷基铵盐处理的反相载体进行高效液相色谱法，从面包酵母中分离转运核糖核酸（tRNA）。虽然通过该柱的分离似乎是由阴离子交换色谱法和反相色谱法共同作用的结果，但疏水相互作用决定了一种tRNA与另一种tRNA的分离。大量tRNA的色谱分析产生了许多具有不同氨基酸接受活性且交叉污染很少的馏分。在某些情况下，该柱分离出了tRNA苯丙氨酸的几种单核苷酸修饰。使用250×6.2毫米的柱子，已能够对至少100个A260单位的tRNA进行色谱分析，而分辨率不会有严重损失。作为纯化程序的最后一步从该柱分离出的tRNA具有非常高的氨基酸接受活性。
