Molecular Biology and Genomics Research Laboratory, Ramjas College, University of Delhi, New Delhi 110007, India.
Department of Zoology, Kirori Mal College, University of Delhi, New Delhi 110007, India.
Int J Syst Evol Microbiol. 2024 Aug;74(8). doi: 10.1099/ijsem.0.006485.
The present study used whole-genome data to clarify the taxonomic assignment of two closely related species. Genomic information for 10 type strains was available at the time of conducting this analysis. One group of type strains was found to be conspecific, namely Kämpfer . 1999 and Pathom-aree . 2006. The 16S rRNA gene sequences showed 99 % similarity between these type strains. Whole-genome-based comparisons showed that DSM 44343 and DSM 44944 shared 98.07 % average nucleotide identity, 98.29 % average amino acid identity and 84.80 % digital DNA-DNA hybridization values. These values exceeded the threshold values for bacterial species delineation. Further, phylogenomic analysis based on the core genomes of the strains under study confirmed that DSM 44343 and DSM 44944 formed a monophyletic clade. Based on this evidence, we propose the reclassification of Pathom-aree . 2006 as a later heterotypic synonym of Kämpfer . 1999.
本研究利用全基因组数据阐明了两个密切相关的种的分类归属。在进行这项分析时，有 10 株模式株的基因组信息可用。一组模式株被发现为同种，即 1999 年的 Kämpfer 和 2006 年的 Pathom-aree。16S rRNA 基因序列显示这些模式株之间具有 99%的相似性。基于全基因组的比较表明，DSM 44343 和 DSM 44944 共享 98.07%的平均核苷酸同一性、98.29%的平均氨基酸同一性和 84.80%的数字 DNA-DNA 杂交值。这些值超过了细菌种划分的阈值。此外，基于所研究菌株的核心基因组的系统基因组分析证实，DSM 44343 和 DSM 44944 形成了一个单系分支。基于这些证据，我们建议将 2006 年的 Pathom-aree 重新分类为 1999 年的 Kämpfer 的后异名。
