Pathom-Aree Wasu, Nogi Yuichi, Sutcliffe Iain C, Ward Alan C, Horikoshi Koki, Bull Alan T, Goodfellow Michael
Division of Biology, King George VI Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):1123-1126. doi: 10.1099/ijs.0.64132-0.
The taxonomic status of an actinomycete isolated from sediment collected from the Mariana Trench was established using a combination of genotypic and phenotypic data. Isolate MT8T had chemotaxonomic and morphological properties consistent with its classification in the genus Williamsia, and formed a distinct phyletic line in the 16S rRNA gene tree together with the type strain of Williamsia muralis. The isolate was readily distinguished from the latter, and from representatives of other Williamsia species, using DNA-DNA relatedness and phenotypic criteria. Predominant cellular fatty acids were oleic, palmitic and tuberculostearic acids and a hexadecenoic acid. The DNA G+C content was 65.2 mol%. It is apparent that the isolate belongs to a novel species of Williamsia. Strain MT8T (=DSM 44944T=NCIMB 14085T) was thus considered to be the type strain of a novel species in the genus Williamsia, for which the name Williamsia marianensis sp. nov. is proposed.
利用基因型和表型数据相结合的方法，确定了从马里亚纳海沟沉积物中分离出的一株放线菌的分类地位。分离株MT8T具有化学分类学和形态学特性，与其在Williamsia属中的分类一致，并在16S rRNA基因树中与壁生Williamsia的模式菌株形成了一个独特的进化分支。利用DNA-DNA相关性和表型标准，该分离株很容易与后者以及其他Williamsia物种的代表区分开来。主要的细胞脂肪酸为油酸、棕榈酸、结核硬脂酸和十六碳烯酸。DNA G+C含量为65.2 mol%。显然，该分离株属于Williamsia属的一个新物种。因此，菌株MT8T（=DSM 44944T=NCIMB 14085T）被认为是Williamsia属一个新物种的模式菌株，为此提出新名称马里亚纳Williamsia（Williamsia marianensis sp. nov.）。
