Department of Biotechnology, Lyallpur Khalsa College, Mohyal Nagar Jalandhar, Punjab, India.
Department of Biochemistry, Central University of Punjab, VPO Ghudda, Bathinda, India.
J Biomater Sci Polym Ed. 2024 Nov;35(16):2506-2527. doi: 10.1080/09205063.2024.2384801. Epub 2024 Aug 13.
essential oil is utilized in outmoded medicine, therapeutics, and the cosmetic industries. However, the extreme volatility, oxidation susceptibility, and instability of this oil restricts its application. Thus, encapsulation is a more effective method of shielding this oil from unfavorable circumstances. The creation of oil/water (O/W) nanoemulsions based on essential oil (CEO), known as CNE ( essential oil nanoemulsions), and an assessment of its biological potential were the goals of this work. UV, fluorescence, and FT-IR methods were used for physiological characterization. Biological activities, including anti-inflammatory, anti-diabetic, and anti-cancer effects. Studies on the pharmacokinetics of CNE were conducted. CNEs encapsulation efficiency was found to be 92%. The CNE nanoemulsions had a spherical shape with polydispersity index of 0.531, size of 200 nm, and a zeta potential of -35.9 mV. Even after being stored at various temperatures for 50 days, CNE nanoemulsions remained stable. Numerous tests were used to determine the antioxidant capacity of CNE, and the following IC50 values (µl/mL) were found: iron chelating assay: 18, hydroxyl radical scavenging: 37, and nitric oxide radical scavenging activity: 58. The percentage of HeLa cells that remained viable after being treated with CNE was 41% at a higher dose of 1 µl. CNE inhibited α-amylase in a dose-dependent manner, with 72% inhibition at its higher dose of 250 µL. Research on the kinetics of drugs showed that nanoemulsions showed Higuchi pattern. This research showed potential use of oil-based nanoemulsions in the food, cosmetic, and pharmaceutical industries.
精油在传统医学、治疗学和化妆品行业中得到应用。然而，这种油的挥发性强、氧化敏感性高、不稳定性强，限制了其应用。因此，封装是保护这种油免受不利环境影响的更有效方法。本工作旨在制备基于精油（CEO）的油/水（O/W）纳米乳（称为 CNE，精油纳米乳），并评估其生物学潜力。采用 UV、荧光和 FT-IR 方法进行生理特性分析。研究了其生物活性，包括抗炎、抗糖尿病和抗癌作用。还研究了 CNE 的药代动力学。结果表明，CNE 的包封效率为 92%。CNE 纳米乳呈球形，多分散指数为 0.531，粒径为 200nm，zeta 电位为-35.9mV。即使在不同温度下储存 50 天，CNE 纳米乳仍保持稳定。采用多种方法测定 CNE 的抗氧化能力，得到以下 IC50 值（µl/mL）：铁螯合试验：18，羟基自由基清除率：37，一氧化氮自由基清除活性：58。在较高剂量 1µl 时，CNE 处理后的 HeLa 细胞存活率为 41%。CNE 对α-淀粉酶呈剂量依赖性抑制，在较高剂量 250µL 时抑制率为 72%。药物动力学研究表明，纳米乳呈现 Higuchi 模式。这项研究表明，油基纳米乳在食品、化妆品和制药行业具有潜在的应用前景。
