Eid Ahmad M, Al-Hawari Haneen, Nazzal Shahd, Khudarieh Samera
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, Palestine.
BMC Complement Med Ther. 2025 Feb 11;25(1):47. doi: 10.1186/s12906-025-04804-2.
Since ancient times, plants with medicinal properties have played a crucial role in the prevention and treatment of various diseases. The current study focuses on the formulation and assessment of Vitis vinifera (V. vinifera) oil nanoemulgel, exploring its potential antimicrobial, anticancer, and anti-inflammatory properties.
The incorporation of Vitis vinifera oil into a nanoemulsion was achieved through the self-nanoemulsifying technique, utilizing Tween 80 and Span 80 as emulsifying agents. The addition of Carbopol hydrogel to the nanoemulsion resulted in the formation of a nanoemulgel. The subsequent evaluation focused on the following properties: rheology, polydispersity index (PDI), zeta potential, and antimicrobial potentials against seven microbial strains, as well as anticancer activities involving B16-F1, Hep-3B, LX-2, and HeLa cell lines, along with anti-inflammatory activities.
The optimal nanoemulsion formulation had a particle size of 173.19 nm and a low PDI of 0.129. Similarly, the nanoemulgel had a particle size of less than 200 nm and a PDI below 0.15. Its zeta potential was less than - 35 mV, and it exhibited pseudoplastic rheological behavior. V. vinifera oil nanoemulgel demonstrated notable antimicrobial activity against MRSA, P. vulgaris, and K. pneumoniae, with inhibition zones of 27 ± 1.6 mm, 39 ± 2.2 mm, and 24 ± 1.3 mm, respectively. Additionally, it showed enhanced anticancer activity against HeLa, LX-2, B16-F1, and Hep-3B cancer cell lines, with IC values of 28.84, 56.23, 151.35, and 66.06 µg/mL, respectively.
These findings show that the nanoemulgel had enhanced activity compared to the oil. Additionally, the nanoemulgel inhibited both COX-1 and COX-2, showing selectivity towards COX-1. This shows the potential of using nanotechnology in the development of pharmaceutical dosage forms.
自古以来，具有药用特性的植物在各种疾病的预防和治疗中发挥了关键作用。当前的研究聚焦于酿酒葡萄（V. vinifera）油纳米乳凝胶的制备与评估，探索其潜在的抗菌、抗癌和抗炎特性。
通过自纳米乳化技术，利用吐温80和司盘80作为乳化剂，将酿酒葡萄籽油掺入纳米乳液中。向纳米乳液中添加卡波姆水凝胶导致形成纳米乳凝胶。随后的评估集中在以下特性上：流变学、多分散指数（PDI）、zeta电位、对七种微生物菌株的抗菌潜力，以及涉及B16-F1、Hep-3B、LX-2和HeLa细胞系的抗癌活性以及抗炎活性。
最佳纳米乳液配方的粒径为173.19纳米，低PDI为0.129。同样，纳米乳凝胶的粒径小于200纳米，PDI低于0.15。其zeta电位小于 - 35毫伏，并且表现出假塑性流变行为。酿酒葡萄籽油纳米乳凝胶对耐甲氧西林金黄色葡萄球菌、普通变形杆菌和肺炎克雷伯菌表现出显著的抗菌活性，抑菌圈分别为27±1.6毫米、39±2.2毫米和24±1.3毫米。此外，它对HeLa、LX-2、B16-F1和Hep-3B癌细胞系显示出增强的抗癌活性，IC值分别为28.84、56.23、151.35和66.06微克/毫升。
这些发现表明，与油相比，纳米乳凝胶具有增强的活性。此外，纳米乳凝胶抑制COX-1和COX-2，对COX-1表现出选择性。这显示了在药物剂型开发中使用纳米技术的潜力。
