Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Department of Chemistry, Faculty of Science, Gonbad Kavous University, Gonbad-e Kavus, Iran.
J Biomater Sci Polym Ed. 2024 Dec;35(17):2660-2681. doi: 10.1080/09205063.2024.2386787. Epub 2024 Aug 5.
The main focus of this study was to create a stable and efficient nanoemulsion (NE) using essential oil (EO). Various factors affecting the NE's stability were optimized including oil %, Tween 80%, time of sonication, and its accelerated stability was examined. The research also considered the antibacterial, antifungal, and larvicidal effects of the optimized NE (B10). The optimum NE stood out for its stability, featuring a particle size of 33.15 ± 0.32 nm. Analysis IR spectroscopy confirmed successful EO encapsulation in B10. The formulation remained stable for six months, with B10 showing significantly higher antibacterial and antifungal potency compared to the pure oil. When samples were subjected to tests against , B10 exhibited a MIC value of 62.5 mg/mL, whereas the pure oil showed a MIC value of 250 mg/mL. This indicates that the B10 formulation was 50 times more effective than the EO. In terms of antibacterial activity against , the MIC value was 0.256 mg/mL for B10 and 4 mg/mL for the EO. Also, pure oil and B10 displayed larvicidal effects against (Walker) larvae, with B10 eliminating 95.2% of larvae in 48 h. Overall, stable and optimum NE with its strong antimicrobial qualities, shows promise as an effective fungicide and insecticide.
本研究的主要重点是使用精油（EO）创建稳定且高效的纳米乳剂（NE）。优化了影响 NE 稳定性的各种因素，包括油%、吐温 80%、超声时间，还检查了其加速稳定性。研究还考虑了优化的 NE（B10）的抗菌、抗真菌和杀幼虫作用。最佳 NE 的特点是稳定性高，粒径为 33.15±0.32nm。IR 光谱分析证实了 EO 在 B10 中的成功包封。该配方在六个月内保持稳定，B10 显示出比纯油更高的抗菌和抗真菌效力。当样品经过针对 的测试时，B10 表现出 62.5mg/mL 的 MIC 值，而纯油表现出 250mg/mL 的 MIC 值。这表明 B10 配方比 EO 有效 50 倍。在针对 的抗菌活性方面，B10 的 MIC 值为 0.256mg/mL，而 EO 的 MIC 值为 4mg/mL。此外，纯油和 B10 对 （Walker）幼虫表现出杀幼虫作用，B10 在 48 小时内消除了 95.2%的幼虫。总的来说，具有强抗菌特性的稳定且最佳的 NE 有望成为有效的杀真菌剂和杀虫剂。
