Xu Luping, Bai Xingjian, Joong Oh Eun
Department of Food Science, Purdue University, West Lafayette, IN, USA.
Whistler Center for Carbohydrate Research, Purdue University, West Lafayette, IN, USA.
Crit Rev Biotechnol. 2025 May;45(3):491-508. doi: 10.1080/07388551.2024.2385996. Epub 2024 Aug 13.
Yeast has been established as a versatile platform for expressing functional molecules, owing to its well-characterized biology and extensive genetic modification tools. Compared to prokaryotic systems, yeast possesses advanced cellular mechanisms that ensure accurate protein folding and post-translational modifications. These capabilities are particularly advantageous for the expression of human-derived functional proteins. However, designing yeast strains as an expression platform for proteins requires the integration of molecular and cellular functions. By delving into the complexities of yeast-based expression systems, this review aims to empower researchers with the knowledge to fully exploit yeast as a functional platform to produce a diverse range of proteins. This review includes an exploration of the host strains, gene cassette structures, as well as considerations for maximizing the efficiency of the expression system. Through this in-depth analysis, the review anticipates stimulating further innovation in the field of yeast biotechnology and protein engineering.
由于酵母具有特征明确的生物学特性和广泛的基因改造工具，它已成为表达功能分子的通用平台。与原核系统相比，酵母拥有先进的细胞机制，可确保蛋白质准确折叠和进行翻译后修饰。这些能力对于表达人源功能蛋白尤为有利。然而，将酵母菌株设计为蛋白质表达平台需要整合分子和细胞功能。通过深入研究基于酵母的表达系统的复杂性，本综述旨在为研究人员提供知识，使其能够充分利用酵母作为功能平台来生产多种蛋白质。本综述包括对宿主菌株、基因盒结构的探索，以及提高表达系统效率的考虑因素。通过这种深入分析，本综述预计将激发酵母生物技术和蛋白质工程领域的进一步创新。
