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phaC 突变对稻黄单胞菌 pv.oryzae 碳源供应、胞外多糖产生和致病性的影响。

Effect of mutation of phaC on carbon supply, extracellular polysaccharide production, and pathogenicity of Xanthomonas oryzae pv. oryzae.

机构信息

College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, 570228, China.

College of Tropical Crops, Hainan University, Haikou, 570228, China.

出版信息

Sci Rep. 2024 Aug 13;14(1):18781. doi: 10.1038/s41598-024-69621-y.

DOI:10.1038/s41598-024-69621-y
PMID:39138326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11322296/
Abstract

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight in rice. Polyhydroxyalkanoates (PHAs) consitute a diverse group of biopolyesters synthesized by bacteria under nutrient-limited conditions. The phaC gene is important for PHA polymerization. We investigated the effects of phaC gene mutagensis in Xoo strain PXO99. The phaC gene knock-out mutant exhibited reduced swarming ability relative to that of the wild-type. Under conditions where glucose was the sole sugar source, extracellular polysaccharide (EPS) production by ΔphaC declined by 44.8%. ΔphaC showed weak hypersensitive response (HR) induction in the leaves of non-host Nicotiana tabacum, concomitant with downregulation of hpa1 gene expression. When inoculated in rice leaves by the leaf-clipping method, ΔphaC displayed reduced virulence in terms of lesion length compared with the wild-type strain. The complemented strain showed no significant difference from the wild-type strain, suggesting that the deletion of phaC in Xoo induces significant alterations in various physiological and biological processes. These include bacterial swarming ability, EPS production, transcription of hrp genes, and glucose metabolism. These changes are intricately linked to the energy utilization and virulence of Xoo during plant infection. These findings revealed involvement of phaC in Xoo is in the maintaining carbon metabolism by functioning in the PHA metabolic pathway.

摘要

稻黄单胞菌（Xanthomonas oryzae pv. oryzae，Xoo）引起水稻细菌性条斑病。聚羟基脂肪酸酯（PHA）是一类由细菌在营养受限条件下合成的生物聚酯。phaC 基因对于 PHA 聚合非常重要。我们研究了 phaC 基因突变对 Xoo 菌株 PXO99 的影响。phaC 基因敲除突变体的泳动能力相对于野生型明显降低。在葡萄糖是唯一糖源的条件下，ΔphaC 的胞外多糖（EPS）产量下降了 44.8%。ΔphaC 在非宿主烟草叶片中诱导的超敏反应（HR）较弱，同时 hpa1 基因表达下调。用叶夹接种法接种水稻叶片时，ΔphaC 的病斑长度比野生型菌株的毒力降低。与野生型菌株相比，补菌与野生型菌株没有显著差异，表明 Xoo 中 phaC 的缺失会导致多种生理和生物学过程的显著改变。这些改变包括细菌泳动能力、EPS 产生、hrp 基因转录和葡萄糖代谢。这些变化与 Xoo 在植物感染过程中的能量利用和毒力密切相关。这些发现表明，phaC 在 PHA 代谢途径中发挥作用，参与了 Xoo 的碳代谢维持。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f72/11322296/07aba58e3063/41598_2024_69621_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f72/11322296/813495358840/41598_2024_69621_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f72/11322296/f2839e7a0192/41598_2024_69621_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f72/11322296/83e77b7c6bad/41598_2024_69621_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f72/11322296/5fa7e8893ac8/41598_2024_69621_Fig13_HTML.jpg

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