Qi Yue, Wang Chen, Lang Haoyu, Wang Yueyi, Wang Xiaofei, Zheng Hao, Lu Yuan
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Synth Syst Biotechnol. 2024 Jul 18;9(4):853-860. doi: 10.1016/j.synbio.2024.07.003. eCollection 2024 Dec.
, a parasite that parasitizes and reproduces in the gut of honeybees, has become a serious threat to the global apiculture industry. RNA interference (RNAi) technology can be used to inhibit growth by targeting silencing the thioredoxin reductase (TrxR) in . However, suitable carriers are one of the reasons limiting the application of RNAi due to the easy degradation of dsRNA in honeybees. As a vesicle composed of a lipid bilayer, liposomes are a good carrier for nucleic acid delivery, but studies in honeybees are lacking. In this study, liposomes were used for double-stranded RNA (dsRNA) dsTrxR delivery triggering RNAi to inhibit the growth in honeybees. Compared to naked dsTrxR, liposome-dsTrxR reduced numbers in the midgut and partially restored midgut morphology without affecting bee survival and gut microbial composition. The results of this study confirmed that liposomes could effectively protect dsRNA from entering the honeybee gut and provide a reference for using RNAi technology to suppress honeybee pests and diseases.
一种寄生于蜜蜂肠道并在其中繁殖的寄生虫，已对全球养蜂业构成严重威胁。RNA干扰（RNAi）技术可通过靶向沉默该寄生虫中的硫氧还蛋白还原酶（TrxR）来抑制其生长。然而，由于双链RNA（dsRNA）在蜜蜂体内易降解，合适的载体是限制RNAi应用的原因之一。脂质体作为一种由脂质双层组成的囊泡，是核酸递送的良好载体，但在蜜蜂中的研究较少。在本研究中，脂质体用于递送双链RNA（dsRNA）dsTrxR以触发RNAi，从而抑制蜜蜂体内该寄生虫的生长。与裸露的dsTrxR相比，脂质体-dsTrxR降低了中肠内该寄生虫的数量，并部分恢复了中肠形态，且不影响蜜蜂的存活和肠道微生物组成。本研究结果证实脂质体可有效保护dsRNA进入蜜蜂肠道，并为利用RNAi技术抑制蜜蜂病虫害提供参考。
