Hu Yingfan, Liu Xiaomin, Song Yu, Zhang Yan, Li Wei, Zhang Lele, Wang Anqi, Su Qian, Yang Zhiyong, Zou Liang
School of Preclinical Medicine Chengdu University Chengdu Sichuan China.
State Key Laboratory of Quality Research in Chinese Medicine University of Macau Macao China.
Food Sci Nutr. 2024 Apr 26;12(8):5426-5441. doi: 10.1002/fsn3.4193. eCollection 2024 Aug.
Golden buckwheat, also called (D. Don) H. Hara, is a plant of the genus Buckwheat in the buckwheat family. The aim of this study was to screen the bioactive ingredients of golden buckwheat extract and investigate the protective effect on acute lung injury (ALI). The ethyl acetate extract (EAE) was identified as the active fraction in LPS-induced RAW264.7 cells, with gallic acid, proanthocyanidin B2, and epicatechin at 0.0563%, 0.3707%, and 0.3868%, respectively. At the same time, 20 compounds (mainly flavonoids and organic acids) were identified by UPLC-Q-Exactive Orbitrap-HRMS in EAE. Furthermore, the EAE reduced lung histopathology scores in mice with ALI, decreased the dry-to-wet weight ratio of lung tissue, and significantly inhibited the concentrations of IL-1β, TNFα, and IL-6 in bronchoalveolar lavage fluid (BALF). It also reduced the number of leukocytes, decreased the activity of MPO in lung tissue, and inhibited the levels of TLR4/NLRP3 pathway mRNA and protein in lung tissue. Our study indicated that golden buckwheat as a source of functional food prevents or treats associated lung diseases by modulating the activation of the TLR4/NLRP3 signaling pathway.
金荞麦，也称为（D. Don）H. Hara，是蓼科荞麦属的一种植物。本研究的目的是筛选金荞麦提取物的生物活性成分，并研究其对急性肺损伤（ALI）的保护作用。乙酸乙酯提取物（EAE）被确定为脂多糖诱导的RAW264.7细胞中的活性组分，其中没食子酸、原花青素B2和表儿茶素的含量分别为0.0563%、0.3707%和0.3868%。同时，通过超高效液相色谱-四极杆-静电场轨道阱高分辨质谱（UPLC-Q-Exactive Orbitrap-HRMS）在EAE中鉴定出20种化合物（主要为黄酮类和有机酸）。此外，EAE降低了ALI小鼠的肺组织病理学评分，降低了肺组织的干湿重比，并显著抑制了支气管肺泡灌洗液（BALF）中IL-1β、TNFα和IL-6的浓度。它还减少了白细胞数量，降低了肺组织中MPO的活性，并抑制了肺组织中TLR4/NLRP3通路mRNA和蛋白的水平。我们的研究表明，金荞麦作为一种功能性食品来源，通过调节TLR4/NLRP3信号通路的激活来预防或治疗相关肺部疾病。
