State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.
Gansu Engineering Research Center of Medical Collagen, P. R. China.
J Mater Chem B. 2024 Sep 11;12(35):8757-8766. doi: 10.1039/d4tb00537f.
Skin aging is influenced by both external environmental factors and intrinsic biological mechanisms. Traditional microsphere implants aim to rejuvenate aging skin through collagen regeneration, yet their non-biodegradability and risk of granuloma formation often limit their effectiveness. In this study, we developed novel, injectable, highly bioactive, and degradable collagen-chitosan double-crosslinked composite microspheres for skin rejuvenation. The microspheres demonstrated excellent injectability, requiring an injection force of only 0.9 N, and significant biodegradability, effectively degraded in solutions containing phosphate buffer, type I collagenase, and pepsin. In addition, the microspheres exhibited excellent biocompatibility and bioactivity, significantly promoting the proliferation, adhesion, and migration of human foreskin fibroblast-1 (HFF-1) cells. In a photoaged mouse skin model, the implantation of microspheres significantly enhanced dermal density and skin elasticity while reducing transepidermal water loss. Importantly, the implant promoted the regeneration of collagen fibers. This study suggests that collagen-chitosan double-crosslinked composite microspheres hold significant potential for skin rejuvenation treatments.
皮肤老化受外部环境因素和内在生物学机制的影响。传统的微球植入物旨在通过胶原蛋白再生来使老化的皮肤恢复活力，但它们不可生物降解和形成肉芽肿的风险常常限制了它们的效果。在这项研究中，我们开发了新型的、可注射的、高生物活性的、可降解的胶原-壳聚糖双重交联复合微球，用于皮肤年轻化。微球表现出优异的可注射性，仅需 0.9 N 的注射力，并且具有显著的可生物降解性，可在含有磷酸盐缓冲液、I 型胶原酶和胃蛋白酶的溶液中有效降解。此外，微球表现出优异的生物相容性和生物活性，显著促进人包皮成纤维细胞-1（HFF-1）细胞的增殖、黏附和迁移。在光老化小鼠皮肤模型中，微球的植入显著增加了真皮密度和皮肤弹性，同时减少了经表皮水分流失。重要的是，植入物促进了胶原纤维的再生。这项研究表明，胶原-壳聚糖双重交联复合微球在皮肤年轻化治疗中具有重要的应用潜力。
