Wang Feng, Wang Mingbo, She Zhending, Fan Kunwu, Xu Cheng, Chu Bin, Chen Changsheng, Shi Shengjun, Tan Rongwei
Department of Plastic Surgery and Burns, Shenzhen Second People's Hospital, Shenzhen 518035, PR China.
Key Laboratory of Biomedical Materials and Implants, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, PR China.
Mater Sci Eng C Mater Biol Appl. 2015;52:155-62. doi: 10.1016/j.msec.2015.03.013. Epub 2015 Mar 10.
Inspired from the sophisticated bilayer structures of natural dermis, here, we reported collagen/chitosan based two-compartment and bi-functional dermal scaffolds. Two functions refer to mediating rapid angiogenesis based on recombinant human vascular endothelial growth factor (rhVEGF) and antibacterial from gentamicin, which were encapsulated in PLGA microspheres. The gentamicin and rhVEGF encapsulated PLGA microspheres were further combined with collagen/chitosan mixtures in low (lower layer) and high (upper layer) concentrations, and molded to generate the two-compartment and bi-functional scaffolds. Based on morphology and pore structure analyses, it was found that the scaffold has a distinct double layered porous and connective structure with PLGA microspheres encapsulated. Statistical analysis indicated that the pores in the upper layer and in the lower layer have great variations in diameter, indicative of a two-compartment structure. The release profiles of gentamicin and rhVEGF exceeded 28 and 49 days, respectively. In vitro culture of mouse fibroblasts showed that the scaffold can facilitate cell adhesion and proliferation. Moreover, the scaffold can obviously inhibit proliferation of Staphylococcus aureus and Serratia marcescens, exhibiting its unique antibacterial effect. The two-compartment and bi-functional dermal scaffolds can be a promising candidate for skin regeneration.
受天然真皮复杂双层结构的启发，在此我们报道了基于胶原蛋白/壳聚糖的双室双功能真皮支架。两种功能分别是基于重组人血管内皮生长因子（rhVEGF）介导快速血管生成以及庆大霉素的抗菌作用，它们被封装在聚乳酸-羟基乙酸共聚物（PLGA）微球中。将封装有庆大霉素和rhVEGF的PLGA微球分别与低浓度（下层）和高浓度（上层）的胶原蛋白/壳聚糖混合物进一步混合，并成型以制备双室双功能支架。基于形态学和孔隙结构分析，发现该支架具有独特的双层多孔且相互连通的结构，其中封装有PLGA微球。统计分析表明，上层和下层的孔隙直径有很大差异，表明其具有双室结构。庆大霉素和rhVEGF的释放曲线分别超过28天和49天。小鼠成纤维细胞的体外培养表明，该支架能够促进细胞黏附和增殖。此外，该支架能够明显抑制金黄色葡萄球菌和粘质沙雷氏菌的增殖，展现出其独特的抗菌效果。这种双室双功能真皮支架有望成为皮肤再生的候选材料。
