壳聚糖-TPP 纳米粒包裹三价 DNA 疫苗候选物对肠道病毒 71 型和柯萨奇病毒 A16 型的免疫原性。
Immunogenicity of trivalent DNA vaccine candidate encapsulated in Chitosan-TPP nanoparticles against EV-A71 and CV-A16.
机构信息
Centre for Virus & Vaccine Research, School of Medical & Life Sciences, Sunway University, Petaling Jaya, 47500, Malaysia.
Department of Biological science, School of Medical & Life Sciences, Sunway University, Petaling Jaya, 47500, Malaysia.
出版信息
Nanomedicine (Lond). 2024;19(21-22):1779-1799. doi: 10.1080/17435889.2024.2372243. Epub 2024 Aug 14.
To develop a trivalent DNA vaccine candidate encapsulated in Chitosan-TPP nanoparticles against hand foot and mouth disease (HFMD) and assess its immunogenicity in mice. Trivalent plasmid carrying the VP1 and VP2 genes of EV-A71, VP1 gene of CV-A16 was encapsulated in Chitosan-TPP nanoparticles through ionic gelation. characterization and immunization studies of the CS-TPP-NPs (pIRES-VP121) were performed. Mice administered with CS-TPP NPs (pIRES-VP121) intramuscularly were observed to have the highest IFN-γ response. Sera from mice immunized with the naked pDNA and CS-TPP-NPs (pIRES-VP121) demonstrated good viral clearance against wild-type EV-A71 and CV-A16 in RD cells. CS-TPP-NPs (pIRES-VP121) could serve as a prototype for future development of multivalent HFMD DNA vaccine candidates.
为了开发一种针对手足口病（HFMD）的三价 DNA 疫苗候选物，将其封装在壳聚糖-TPP 纳米粒子中，并在小鼠中评估其免疫原性。通过离子凝胶化将携带 EV-A71 的 VP1 和 VP2 基因以及 CV-A16 的 VP1 基因的三价质粒封装在壳聚糖-TPP 纳米粒子中。对 CS-TPP-NPs（pIRES-VP121）进行了特性和免疫研究。肌肉内给予 CS-TPP NPs（pIRES-VP121）的小鼠观察到具有最高的 IFN-γ 反应。用裸 pDNA 和 CS-TPP-NPs（pIRES-VP121）免疫的小鼠的血清在 RD 细胞中对野生型 EV-A71 和 CV-A16 显示出良好的病毒清除作用。CS-TPP-NPs（pIRES-VP121）可以作为未来开发多价 HFMD DNA 疫苗候选物的原型。
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