Department of Urology, Yichang Central People's Hospital, The First Clinical Medical College, Three Gorges University, Yichang, Hubei, PR China.
Department of Obstetrics and Gynaecology, Yichang Central People's Hospital, The First Clinical Medical College, Three Gorges University, Yichang, Hubei, PR China.
Aging Male. 2024 Dec;27(1):2391380. doi: 10.1080/13685538.2024.2391380. Epub 2024 Aug 14.
Direct evidence for the relationship between a large prostate (≥80 ml) and androgen receptor/PSA signal remains lacking in benign prostatic hyperplasia (BPH). Our aim is to identify whether the cause of a large prostate is related to progesterone receptor (PGR) androgen receptor (AR), oestrogen receptor α, β (ERα,β) and prostate-specific antigen (PSA).
Surgical specimens of BPH in plasmakinetic resection of the prostate (PKRP) with three groups of different prostate-sizes with mean volumes of 25.97 ml, 63.80 ml, and 122.37 ml were collected for immunohistochemical analysis of the tissue microarray with PGR, AR, PSA and ERs. Rats were castrated and treated with testosterone replacement to explore androgen and PGR, AR and ERs expression levels in the prostate. Quantitative real-time reverse transcription polymerase chain reaction (Rt-PCR) for mRNA detection of above genes was conducted.
Immunoblotting, Rt-PCR and immunohistochemistry assays showed that PGR, PSA, AR, ERα expression levels were positively correlated with prostate size and that ERβ expression levels were negatively correlated with prostate volume. Animal experiments have shown that prostate volume is decreased in castrated rats with decreased PGR, AR, ERα and increased ERβ expression levels.
PGR, AR, ERs signals can be regarded as important factors for large-sized prostates in BPH patients (≥100 ml).
在良性前列腺增生症（BPH）中，仍然缺乏大前列腺（≥80ml）与雄激素受体/PSA 信号之间关系的直接证据。我们的目的是确定大前列腺的原因是否与孕激素受体（PGR）、雄激素受体（AR）、雌激素受体α、β（ERα、β）和前列腺特异性抗原（PSA）有关。
收集经等离子体前列腺剜除术（PKRP）治疗的 BPH 手术标本，分为三组，前列腺体积分别为 25.97ml、63.80ml 和 122.37ml，进行组织微阵列的免疫组织化学分析，检测 PGR、AR、PSA 和 ERs。对大鼠进行去势并给予睾酮替代治疗，以探讨前列腺中雄激素和 PGR、AR 和 ERs 的表达水平。进行上述基因的定量实时逆转录聚合酶链反应（Rt-PCR）检测 mRNA 表达。
免疫印迹、Rt-PCR 和免疫组织化学检测结果表明，PGR、PSA、AR、ERα 的表达水平与前列腺体积呈正相关，而 ERβ 的表达水平与前列腺体积呈负相关。动物实验表明，去势大鼠的前列腺体积减小，PGR、AR、ERα 表达降低，ERβ 表达升高。
PGR、AR、ERs 信号可作为 BPH 患者（≥100ml）大前列腺的重要因素。
