Department of Pathology, Johns Hopkins University School of Medicine;
Department of Pathology, Johns Hopkins University School of Medicine; Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine.
J Vis Exp. 2024 Jul 26(209). doi: 10.3791/66836.
Oral gavage (OG) with the use of a cannula attached to a syringe is one of the most common methods used to deliver precise dosing of compounds to the stomach of research animals. Unfortunately, this method comes with difficulties for both the operator and the research animal. Studies have shown that OG may lead to complications, including esophagitis, perforation of the esophagus, and inadvertent tracheal drug administration. In addition, OG is associated with increased plasma and fecal corticosterone levels (due to stress), altered blood pressure, and increased heart rate, which could negatively influence or bias study results. A previously developed alternative method termed micropipette-guided drug administration (MDA) incentivizes the animal to consume treatments readily in a minimally invasive manner. Herein, we present examples of the use of the MDA technique with treatments reconstituted in different vehicles and demonstrate effective delivery of the varied treatments to multiple different mouse strains. We further demonstrate that MDA is a technique that decreases the timing and invasiveness of drug administration and does not affect the gut microbiome composition as assessed by quantitative analysis of core gut microbial species. Overall, MDA may offer a less stressful and effective alternative to OG.
经口灌胃（OG）是一种将化合物精确剂量输送到研究动物胃部的常用方法，使用附有注射器的插管进行。不幸的是，这种方法对操作人员和研究动物都存在困难。研究表明，OG 可能导致并发症，包括食管炎、食管穿孔和意外气管内药物给药。此外，OG 与血浆和粪便皮质酮水平升高（由于应激）、血压改变和心率增加有关，这可能会对研究结果产生负面影响或产生偏差。先前开发的一种替代方法，称为微量移液器引导药物给药（MDA），鼓励动物以微创的方式轻松接受治疗。在此，我们展示了 MDA 技术在不同载体中配制的治疗方法的应用实例，并证明了对多种不同的小鼠品系进行了有效的治疗。我们进一步证明 MDA 是一种可以减少药物给药时间和侵入性的技术，并且不会影响肠道微生物组组成，因为通过核心肠道微生物物种的定量分析来评估。总体而言，MDA 可能提供一种较 OG 更具压力和有效的替代方法。
