Marinho Marcelo Augusto Germani, da Silva Marques Magno, de Oliveira Vian Camila, de Moraes Vaz Batista Filgueira Daza, Horn Ana Paula
Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS 96210-900, Brazil; Laboratório de Cultura Celular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS 96210-900, Brazil.
Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS 96210-900, Brazil; Laboratório de Neurociências, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS 96210-900, Brazil.
Toxicol In Vitro. 2024 Oct;100:105917. doi: 10.1016/j.tiv.2024.105917. Epub 2024 Aug 12.
Glioblastoma is a malignant neoplasm that develops in the central nervous system and is characterized by high rates of cell proliferation and invasion, presenting resistance to treatments and a poor prognosis. Photodynamic therapy (PDT) is a therapeutic modality that can be applied in oncological cases and stands out for being less invasive. Photosensitizers (PS) of natural origin gained prominence in PDT. Curcumin (CUR) is a natural compound that has been used in PDT, considered a promising PS. In this work, we evaluated the effects of PDT-mediated CUR and near-infrared radiation (NIR) in glioblastoma cells. Through trypan blue exclusion analysis, we chose the concentration of 5 μM of CUR and the dose of 2 J/cm of NIR that showed better responses in reducing the viable cell number in the C6 cell line and did not show cytotoxic/cytostatic effects in the HaCat cell line. Our results show that there is a positive interaction between CUR and NIR as a PDT model since there was an increase in ROS levels, a decrease in cell proliferation, increase in cytotoxicity with cell death by autophagy and necrosis, in addition to the presence of oxidative damage to proteins. These results suggest that the use of CUR and NIR is a promising strategy for the antitumor application of PDT.
胶质母细胞瘤是一种发生于中枢神经系统的恶性肿瘤，其特征是细胞增殖和侵袭率高，对治疗具有抗性且预后不良。光动力疗法（PDT）是一种可应用于肿瘤病例的治疗方式，因其侵入性较小而备受关注。天然来源的光敏剂（PS）在光动力疗法中崭露头角。姜黄素（CUR）是一种已用于光动力疗法的天然化合物，被认为是一种有前景的光敏剂。在这项工作中，我们评估了光动力疗法介导的姜黄素和近红外辐射（NIR）对胶质母细胞瘤细胞的影响。通过台盼蓝排斥分析，我们选择了5μM的姜黄素浓度和2J/cm的近红外辐射剂量，它们在降低C6细胞系中的活细胞数量方面显示出更好的反应，并且在HaCat细胞系中未显示出细胞毒性/细胞抑制作用。我们的结果表明，作为一种光动力疗法模型，姜黄素和近红外辐射之间存在积极的相互作用，因为活性氧水平升高、细胞增殖减少、细胞毒性增加以及细胞通过自噬和坏死死亡，此外还存在蛋白质氧化损伤。这些结果表明，使用姜黄素和近红外辐射是光动力疗法抗肿瘤应用的一种有前景的策略。
