姜黄素敏化对人多形性胶质母细胞瘤细胞的影响。
Effects of Photosensitization of Curcumin in Human Glioblastoma Multiforme Cells.
机构信息
Faculty of Medicine, Wroclaw Medical University, Wroclaw, Poland.
Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wroclaw, Poland.
出版信息
In Vivo. 2019 Nov-Dec;33(6):1857-1864. doi: 10.21873/invivo.11679.
Abstract
BACKGROUND/AIM: There is no satisfactory treatment of glioblastoma multiforme, a highly invasive brain tumor. The aim of this study was to analyze the cytotoxic effects of curcumin (CUR) alone and as a photosensitizer on glioblastoma cells.
MATERIALS AND METHODS
The SNB-19 cells where incubated for 2 and 24 h with 5-200 mM of CUR. The cells were radiated with blue light (6 J/cm) and compared to non-irradiated ones. The effects of treatment were assessed by measuring mitochondrial activity with the MTT method and apoptosis progression by flow cytometry. To investigate CUR uptake, fluorescence imaging of cells was performed.
RESULTS
Photosensitization of CUR decreased the EC 6.3 times when the incubation time was 2 h and over 90% of cells underwent apoptosis. The study of the uptake of CUR showed that during the 2 h, CUR was placed in the entire cytoplasm, and over time, its amount decreased and localized in the subcellular compartments.
CONCLUSION
CUR is a promising medicament that can be used as a photosensitizer in photodynamic therapy for glioma treatment.
摘要
背景/目的：多形性胶质母细胞瘤是一种高度侵袭性的脑肿瘤，目前尚无满意的治疗方法。本研究旨在分析姜黄素（CUR）单独使用和作为光敏剂对神经胶质瘤细胞的细胞毒性作用。
材料和方法
将 SNB-19 细胞与 5-200 mM 的 CUR 孵育 2 和 24 小时。用蓝光（6 J/cm）照射细胞，并与未照射的细胞进行比较。用 MTT 法测量线粒体活性和流式细胞术检测细胞凋亡来评估治疗效果。为了研究 CUR 的摄取，对细胞进行荧光成像。
结果
CUR 的光致敏作用使孵育 2 小时时的 EC 降低了 6.3 倍，超过 90%的细胞发生了凋亡。CUR 摄取的研究表明，在 2 小时内，CUR 被放置在整个细胞质中，随着时间的推移，其数量减少并定位于亚细胞区室中。
结论
CUR 是一种很有前途的药物，可作为光动力疗法治疗神经胶质瘤的光敏剂。
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