Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, PR China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Anal Chim Acta. 2024 Sep 1;1320:343017. doi: 10.1016/j.aca.2024.343017. Epub 2024 Jul 24.
Hydrogen peroxide (HO) plays a vital role in human health and have been regarded as a crucial analyte in metabolic processes, redox transformations, foods research and medical fields. Especially, the long-time and excessive digestion of HO may even cause severe diseases. Although conventional instrumental methods and nanozymes-based colorimetric methods have been developed to accomplish the quantitative analysis of HO, the drawbacks of instrument dependence, cost-effectiveness, short lifespan, non-portable and unsustainable detection efficacies will limit their applications in different detection scenarios.
Herein, to address these challenges, we have proposed a novel strategy for nanozyme (RuO) hydrogel preparation by the solid support from cross-linked polyvinyl alcohol (PVA) and chitosan (CS) to both inherit the dominant peroxidase-like (POD) activity and protect the RuO from losing efficacies. Taking advantages from the hydrogel, the encapsulated RuO were further prepared as the regularly spherical beads (PCRO) to exhibit the sustainable, recyclable, and robust catalysis. Moreover, the intrinsic color interferences which originated from RuO can be avoided by the encapsulation strategy to promote the detection accuracy. Meanwhile, the high mechanical strength of PCRO shows the high stability, reproducibility, and cyclic catalysis to achieve the recyclable detection performance and long lifetime storage (40 days), which enables the sensitively detection of HO with the detection limit as lower to 15 μM and the wide detection linear range from 0.025 to 1.0 mM.
On the basis of the unique properties, PCRO has been further adopted to construct a smartphone detection platform to realize the instrument-free and visual analysis of HO in multi-types of milk and real water samples through capturing, processing, and analyzing the RGB values from the colorimetric photographs. Therefore, PCRO with the advanced detection efficacies holds the great potential in achieving the portable and on-site analysis of targets-of-interest.
过氧化氢 (HO) 在人类健康中起着至关重要的作用，并且一直被认为是代谢过程、氧化还原转化、食品研究和医学领域中的关键分析物。特别是，长时间和过量的 HO 消化甚至可能导致严重疾病。尽管已经开发出了传统的仪器方法和基于纳米酶的比色法来完成 HO 的定量分析，但是仪器依赖性、成本效益、寿命短、不可移动和不可持续的检测效果等缺点将限制它们在不同检测场景中的应用。
在这里，为了解决这些挑战，我们提出了一种通过交联聚乙烯醇 (PVA) 和壳聚糖 (CS) 的固体支撑物来制备纳米酶 (RuO) 水凝胶的新策略，以继承其主要的过氧化物酶样 (POD) 活性并保护 RuO 不失活。利用水凝胶的优势，进一步将封装的 RuO 制备成规则的球形珠 (PCRO)，以表现出可持续、可回收和强大的催化作用。此外，封装策略可以避免源于 RuO 的固有颜色干扰，以提高检测准确性。同时，PCRO 的高机械强度表现出高稳定性、重现性和循环催化作用，实现了可重复检测性能和长达 40 天的长寿命储存，从而能够实现对 HO 的灵敏检测，检测限低至 15 μM，检测线性范围从 0.025 到 1.0 mM。
基于其独特的性质，PCRO 进一步被用于构建智能手机检测平台，通过捕捉、处理和分析比色照片中的 RGB 值，实现了多种类型的牛奶和实际水样中 HO 的无仪器和可视化分析。因此，具有先进检测效果的 PCRO 在实现目标物的便携式和现场分析方面具有巨大的潜力。
