Guangdong Provincial Key Laboratory on Functional Soft Condensed Matter, Materials and Energy School, Guangdong University of Technology, Panyu District, Guangzhou 510006, China.
Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou 510317, China.
ACS Appl Mater Interfaces. 2024 Jul 31;16(30):39857-39866. doi: 10.1021/acsami.4c07835. Epub 2024 Jul 17.
Total antioxidants play a crucial role in human health, and detection of the total antioxidant capacity (TAC) has broad application prospects in fields such as food safety, environmental assessment, and disease diagnosis. However, a long detection time, cumbersome steps, high cost, reliance on professional equipment, and nonportability still remain significant challenges. In this work, an efficient strategy of point-of-care testing (POCT) of the TAC in body fluids by nanozyme-catalyzed colorimetric paper-based microfluidic sensors is proposed. The paper-based microfluidic sensors coupled with a smartphone can reduce testing costs and provide portability. The nanozyme prepared by the solvothermal method presents Michaelis constants of 0.11 and 0.129 mM for HO and TMB, respectively. A method for immobilizing nanozymes and chromogenic agents on a paper-based microfluidic chip is established. Based on smartphone photography and image grayscale extraction, the TAC can be qualitatively detected with a detection limit and linear range of 33.4 and 50-700 μM, respectively. Furthermore, the proposed sensor can realize the one-step quantitative analysis of the TAC in body fluids (blood, saliva, and sweat) within 15 min. The proposed nanozyme-catalyzed colorimetric paper-based microfluidic sensors presented in this study exhibit promising application prospects in the fields of biochemical analysis and POCT.
总抗氧化剂在人类健康中起着至关重要的作用，总抗氧化能力（TAC）的检测在食品安全、环境评估和疾病诊断等领域具有广阔的应用前景。然而，较长的检测时间、繁琐的步骤、高成本、对专业设备的依赖和非便携性仍然是重大挑战。在这项工作中，提出了一种通过纳米酶催化比色纸基微流控传感器在体液中进行 TAC 的即时检测（POCT）的有效策略。与智能手机结合使用的纸基微流控传感器可以降低检测成本并提供便携性。通过溶剂热法制备的纳米酶对 HO 和 TMB 的米氏常数分别为 0.11 和 0.129mM。建立了一种将纳米酶和显色剂固定在纸基微流控芯片上的方法。基于智能手机摄影和图像灰度提取，可以定性检测 TAC，检测限和线性范围分别为 33.4 和 50-700μM。此外，该传感器可以在 15 分钟内实现体液（血液、唾液和汗液）中 TAC 的一步定量分析。本研究中提出的纳米酶催化比色纸基微流控传感器在生化分析和 POCT 领域具有广阔的应用前景。
