[ promotes the occurrence of esophageal squamous cell carcinoma via an inflammatory microenvironment].

To investigate the role of an inflammatory microenvironment induced by () in the occurrence of esophageal squamous cell carcinoma (ESCC) in mice. A total of 180 C57BL/6 mice were randomly divided into 6 groups, i.e. control group, group, 4NQO group, 4NQO + group, 4NQO + + celecoxib group, and 4NQO + + antibiotic cocktail (ABC, including metronidazole, neomycin, ampicillin, and vancomycin) group, with 30 mice in each group, using the random number table. All mice were normalized by treatment with ABC in drinking water for 2 weeks. In the following 2 weeks, the mice in the control group and the group were given drinking water, while the other 4 groups were treated with 30 μg/ml 4NQO in the drinking water. In weeks 11-12, the mice in the group, the 4NQO + group, the 4NQO + + celecoxib group, and the 4NQO + + ABC group were subjected to ligation of the second molar in oral cavity followed by oral infection thrice weekly for 24 weeks in weeks 11-34. In weeks 13-34, the mice in 4NQO + +celecoxib group and 4NQO + + ABC group were administered with celecoxib and ABC for 22 weeks, respectively. At the end of 34 weeks, gross and microscopic alterations were examined followed by RT-qPCR and immunohistochemistry to examine the expression profiles of inflammatory- and tumor-molecules in esophagi of mice. At 34 weeks, 4NQO treatment alone did not affect the foci of papillary hyperproliferation, diseased area, and the thickness of the esophageal wall, but significantly enhanced the foci of hyperproliferation (median 1.00, ＜0.05) and mild/moderate dysplasia (median 2.00, ＜0.01). In addition, the expression levels of IL-6 [8.35(3.45,8.99)], IL-1β [6.90(2.01,9.72)], TNF-α [12.04(3.31,14.08)], c-myc [2.21(1.80,3.04)], pSTAT3, Ki-67, and pH2AX were higher than those in the control group. The pathological changes of the esophageal mucosa were significantly more overt in the 4NQO + group in terms of the foci of papillary hyperproliferation (median 2.00), diseased area (median 2.51 mm), the thickness of the esophageal wall (median 172.52 μm), the foci of hyperproliferation (median 1.00, ＜0.05), and mild/moderate dysplasia (median 1.00, ＜0.01). In mice of the 4NQO + group, the expression levels of IL-6 [12.27(5.35,22.08)], IL-1β [13.89(10.04,15.96)], TNF-α [19.56(6.07,20.36)], IFN-γ [11.37(8.23,20.07)], c-myc [2.62(1.51,4.25)], cyclin D1 [4.52(2.68,7.83)], nuclear pSTAT3, COX-2, Ki-67, and pH2AX were significantly increased compared with the mice in the control group. In mice of the 4NQO + group, the diseased area, invasive malignant foci as well as pSTAT3 and pH2AX expression were significantly blunted by celecoxib. Treatment with ABC markedly reduced the papillary hyperproliferative foci, invasive malignant foci, and pSTAT3 expression but not pH2AX. promotes the occurrence of esophageal squamous cell carcinoma in mice by inducing an inflammatory microenvironment primed with 4NQO induced DNA damage. Clearance of with ABC or anti-inflammatory intervention holds promise for prevention of esophageal squamous cell malignant pathogenesis via blockage of IL-6/STAT3 signaling and amelioration of inflammation.