Maćkowiak Bartosz, Ostrowska Kamila, Kulcenty Katarzyna, Kaźmierska Joanna, Ostapowicz Julia, Nowicka Hanna, Szewczyk Mateusz, Książek Krzysztof, Suchorska Wiktoria M, Golusiński Wojciech
Department of Head and Neck Surgery, Poznan University of Medical Sciences, Poznan, Poland.
Radiobiology Laboratory, The Greater Poland Cancer Centre, Poznan, Poland.
Rep Pract Oncol Radiother. 2024 Jun 6;29(2):148-154. doi: 10.5603/rpor.99676. eCollection 2024.
Head and neck squamous carcinoma (HNSC) is the sixth most common neoplasm, with a 40-50% overall survival rate. HNSC standard treatment depends on tumor size, metastasis or human papillomavirus (HPV) status including surgery, chemotherapy, and radiotherapy. The last two may lead to defects in the tumor microenvironment and cancer cell biology as disorders in DNA damage repair systems. Here, we evaluate the correlation between single nucleotide polymorphism (SNP) rs2228001 in the gene with the early and late adverse effects of radiotherapy, determine the distribution of the SNP and post-treatment follow-up in HNSC patients.
Head and neck cancer tissues and clinical data were obtained from 79 patients. The SNP of the gene (rs2228001) was evaluated with polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The chi-square test was used to determine the correlation between mutation and adverse effects occurrence.
RESULTS/CONCLUSION: Single nucleotide polymorphism rs2228001 in the gene is correlated with the early adverse effect of skin reaction and the late adverse effect of elevated C-reactive protein (CRP) levels in the HNSC patients.
头颈部鳞状细胞癌（HNSC）是第六大常见肿瘤，总体生存率为40%-50%。HNSC的标准治疗取决于肿瘤大小、转移情况或人乳头瘤病毒（HPV）状态，包括手术、化疗和放疗。后两者可能会导致肿瘤微环境和癌细胞生物学方面的缺陷，如DNA损伤修复系统紊乱。在此，我们评估基因中的单核苷酸多态性（SNP）rs2228001与放疗的早期和晚期不良反应之间的相关性，确定该SNP在HNSC患者中的分布情况以及治疗后的随访情况。
从79例患者中获取头颈部癌组织和临床数据。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术评估基因（rs2228001）的SNP。采用卡方检验确定突变与不良反应发生之间的相关性。
结果/结论：基因中的单核苷酸多态性rs2228001与HNSC患者皮肤反应的早期不良反应以及C反应蛋白（CRP）水平升高的晚期不良反应相关。
