You Ye-Lim, Choi Hyeon-Son
Department of Food Nutrition, Sangmyung University, Hongjimun 2-Gil 20, Jongno-Gu, Seoul, 03016 Republic of Korea.
Food Sci Biotechnol. 2024 Feb 3;33(11):2631-2641. doi: 10.1007/s10068-024-01522-2. eCollection 2024 Aug.
Nootkatone (NK) is an aromatic compound derived from grapefruit. This study aimed to investigate the inhibitory effect of NK on lipid accumulation and its underlying mechanism in adipocytes. NK effectively inhibited adipogenic lipid storage by downregulating C/EBPα and PPARγ, while upregulating KLF2, an early inhibitory factor, downregulating C/EBPβ, an early promoting factor. In addition, NK inhibited the JAK2-STAT signaling pathway by decreasing the phosphorylation of STAT3 and STAT5 in the early adipogenic stage. NK significantly reduced ROS generation while elevating antioxidant enzymes such as catalase and glutathione peroxidase. It activated NRF2-HO-1 signaling, responsible for antioxidant response, by increasing protein levels. Furthermore, NK regulated adipokines, increasing adiponectin and visfatin, while downregulating resistin. Collectively, NK inhibited adipogenic lipid accumulation through the suppression of JAK2-STAT signaling and the augmentation of antioxidant response. This study highlights the potential of NK as an edible agent to alleviate obesity and its associated metabolic diseases.
The online version contains supplementary material available at 10.1007/s10068-024-01522-2.
诺卡酮（NK）是一种源自葡萄柚的芳香族化合物。本研究旨在探讨NK对脂肪细胞脂质积累的抑制作用及其潜在机制。NK通过下调C/EBPα和PPARγ，同时上调早期抑制因子KLF2、下调早期促进因子C/EBPβ，有效抑制脂肪生成性脂质储存。此外，NK在脂肪生成早期通过降低STAT3和STAT5的磷酸化来抑制JAK2-STAT信号通路。NK显著减少活性氧的产生，同时提高过氧化氢酶和谷胱甘肽过氧化物酶等抗氧化酶的水平。它通过增加蛋白质水平激活负责抗氧化反应的NRF2-HO-1信号通路。此外，NK调节脂肪因子，增加脂联素和内脏脂肪素，同时下调抵抗素。总体而言，NK通过抑制JAK2-STAT信号通路和增强抗氧化反应来抑制脂肪生成性脂质积累。本研究突出了NK作为一种可食用剂缓解肥胖及其相关代谢疾病的潜力。
在线版本包含可在10.1007/s10068-024-01522-2获取的补充材料。
