Arakawa H, Maeda M, Tsuji A
J Immunoassay. 1985;6(4):347-61. doi: 10.1080/01971528508063038.
An enzyme immunoassay of 21-deoxycortisol (21-DOF) in plasma and dried blood spotted on filter paper has been developed. 21-DOF was conjugated to horseradish peroxidase by the mixed anhydride method. Separation of free and bound fractions was done by the use of insolubilized antibody, prepared by coating polyacetal beads with purified IgG of goat anti-rabbit IgG serum. The enzyme activity was measured by the fluorophotometric method using 3-(p-hydroxyphenyl) propionic acid and H2O2 as substrates. The sensitivity of the present method was 0.5 pg/tube for 21-DOF. The intra- and interassay coefficients of variation were 3.2 and 8.2, and 7.9 and 9.2% respectively. The present enzyme immunoassay could be applied to mass-screening for congenital adrenal hyperplasia.
已开发出一种用于检测血浆和滤纸上干血斑中21-脱氧皮质醇（21-DOF）的酶免疫测定法。通过混合酸酐法将21-DOF与辣根过氧化物酶偶联。使用通过用山羊抗兔IgG血清的纯化IgG包被聚缩醛珠体制备的不溶性抗体来分离游离和结合部分。使用3-（对羟基苯基）丙酸和H2O2作为底物，通过荧光光度法测量酶活性。本方法对21-DOF的灵敏度为0.5 pg/管。批内和批间变异系数分别为3.2%和8.2%，以及7.9%和9.2%。本酶免疫测定法可应用于先天性肾上腺皮质增生症的大规模筛查。
