Wang Chao, Wang Yida, Zhu Weihang, Tang Qian, Wang Xuekang, Zhang Lu
Department of Spine Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, China.
Heliyon. 2024 Jul 22;10(15):e34755. doi: 10.1016/j.heliyon.2024.e34755. eCollection 2024 Aug 15.
Ossification of the ligamentum flavum (OLF) is the primary etiology of thoracic spinal stenosis. The functional properties of epidural fat (EF), an adipose tissue located in close proximity to ligamentum flavum (LF), have been scarcely investigated. The metabolic state of adipocytes significantly influences their functionality, and exosomes play a pivotal role in intercellular communication. This study aimed to investigate the role of EF-derived exosomes in OLF and characterize their protein profile by proteomics analysis. Our findings demonstrate that exosomes obtained from EF adjacent to OLF possess the ability to enhance osteogenesis of fibroblasts . Furthermore, proteomics analysis revealed metabolic dysfunction in EF adipocytes and identified lactate dehydrogenase A (LDHA) as a potential mediator involved in the development of OLF. This study provides new insights into the pathogenic mechanism underlying OLF and offers a theoretical basis for preventing and treating ligament ossification.
黄韧带骨化（OLF）是胸段脊髓狭窄的主要病因。硬膜外脂肪（EF）是一种紧邻黄韧带（LF）的脂肪组织，其功能特性鲜有研究。脂肪细胞的代谢状态显著影响其功能，而外泌体在细胞间通讯中起关键作用。本研究旨在探讨EF衍生的外泌体在OLF中的作用，并通过蛋白质组学分析表征其蛋白质谱。我们的研究结果表明，从OLF附近的EF获得的外泌体具有增强成纤维细胞成骨的能力。此外，蛋白质组学分析揭示了EF脂肪细胞的代谢功能障碍，并确定乳酸脱氢酶A（LDHA）是参与OLF发生发展的潜在介质。本研究为OLF的发病机制提供了新的见解，并为预防和治疗韧带骨化提供了理论依据。
