Zeb Alam, Ullah Ubaid, Mehmood Arif
Department of Biochemistry, University of Malakand, Chakdara, Pakistan.
Department of Biotechnology, University of Malakand, Chakdara, Pakistan.
Heliyon. 2024 Jul 20;10(15):e34982. doi: 10.1016/j.heliyon.2024.e34982. eCollection 2024 Aug 15.
L. (MC) is a widely consumed vegetable known for its nutritional benefits, as it is a rich source of carotenoids and phenolic compounds. Various cooking methods are use in domestic settings, including microwave cooking. Therefore, it is crucial to investigate the impact of microwave cooking on the bioactive composition of MC. MC fruits were subjected to microwave heat for 5-, 10-, and 15-min. High performance liquid chromatography was used to identify of carotenoids and phenolic compounds, and total bioactive composition and antioxidant assays were conducted using spectroscopic techniques. There were 17 carotenoids and chlorophylls identified in MC fruit, including lutein, violaxanthin, antheraxanthin, pheophytin , and all--β-carotene. The levels of these compounds significantly increased upon exposure to microwave heating. Similarly, 16 phenolic compounds were identified, and their amounts increased during the treatments, except for 3-hydroxyphloretin-6'-hexoside, quercetin-3-(6″-acetyl)-glucoside, petunidin-3-(6″-acetyl)-glucoside and petunidin-3-(6″-acetyl)-glucoside. The sample subjected to microwave treatment for 15 min exhibited the highest concentration of total phenolic compounds (TPC) at 754.5 mg/100g. The total flavonoid content (TFC) reached 94.6 mg/100g after 10 min of treatment. Additionally, the maximum total anthocyanin content, reported as 54.8 mg/L, was observed in the sample exposed to microwave heating for 15 min.
L.(MC)是一种广泛食用的蔬菜,因其营养价值而闻名,因为它富含类胡萝卜素和酚类化合物。家庭环境中使用了各种烹饪方法,包括微波烹饪。因此,研究微波烹饪对MC生物活性成分的影响至关重要。将MC果实进行5分钟、10分钟和15分钟的微波加热。使用高效液相色谱法鉴定类胡萝卜素和酚类化合物,并使用光谱技术进行总生物活性成分和抗氧化测定。在MC果实中鉴定出17种类胡萝卜素和叶绿素,包括叶黄素、紫黄质、花药黄质、脱镁叶绿素和全反式β-胡萝卜素。这些化合物的含量在微波加热后显著增加。同样,鉴定出16种酚类化合物,除3-羟基根皮素-6'-己糖苷、槲皮素-3-(6″-乙酰基)-葡萄糖苷、矮牵牛素-3-(6″-乙酰基)-葡萄糖苷和矮牵牛素-3-(6″-乙酰基)-葡萄糖苷外,它们的含量在处理过程中增加。经15分钟微波处理的样品总酚化合物(TPC)浓度最高,为754.5毫克/100克。处理10分钟后总黄酮含量(TFC)达到94.6毫克/100克。此外,在经15分钟微波加热的样品中观察到最大总花青素含量,为54.8毫克/升。