Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Department of Medicine, Division of Infectious Disease and Immunology, University of Massachusetts Chan Medical School, Worcester, MA, USA.
Methods Mol Biol. 2024;2828:45-55. doi: 10.1007/978-1-0716-4023-4_5.
Multiphoton intravital microscopy (MP-IVM) is an imaging technique used for the observation of living organisms at a microscopic resolution. The tissue of interest is exposed through a window allowing imaging of cells in real time. Using MP-IVM, the temporospatial kinetics of leukocyte transendothelial migration can be visualized and quantitated using reporter mice and cell-specific fluorophore-conjugated monoclonal antibodies to track the leukocytes within and outside of vascular beds. Here we describe a method used to study neutrophil transendothelial migration and blood-brain barrier permeability in a mouse model of herpes simplex virus I (HSV) encephalitis.
多光子活体显微镜（MP-IVM）是一种用于以微观分辨率观察活体的成像技术。通过一个窗口使感兴趣的组织暴露，从而可以实时观察细胞。使用 MP-IVM，可以可视化白细胞穿越内皮的时空动力学，并使用报告基因小鼠和细胞特异性荧光素偶联的单克隆抗体进行定量，以跟踪血管床内外的白细胞。在此，我们描述了一种用于研究单纯疱疹病毒 I（HSV）脑炎小鼠模型中性粒细胞穿越内皮迁移和血脑屏障通透性的方法。
