Tianjin Second People's Hospital, Tianjin, 300192, China.
Clinical School of the Second People's Hospital, Tianjin Medical University, Tianjin, 300192, China.
Virol J. 2024 Aug 15;21(1):187. doi: 10.1186/s12985-024-02461-4.
Enterovirus 71 (EV-71) has strong neurotropism, and it is the main pathogen causing severe hand, foot, and mouth disease (HFMD). In clinical observations, significant differences were observed in the severity and prognosis of HFMD among children who were also infected with EV-71. Genetic differences among individuals could be one of the important causes of differences in susceptibility to EV-71-induced HFMD. As P-selectin glycoprotein ligand-1 (PSGL-1) is an important receptor of EV-71, the correlation between single-nucleotide polymorphisms (SNPs) in PSGL-1 and the susceptibility to severe HFMD following EV-71 infection is worth studying. Given the role of PSGL-1 in immunity, the correlations between PSGL-1 SNPs and the immune status after EV-71 infection are also worth studying. Meanwhile, PSGL-1 variable number of tandem repeats (VNTR) represents a research hotspot in cardiovascular and cerebrovascular diseases, but PSGL-1 VNTR polymorphism has not been investigated in HFMD caused by EV-71 infection. In this study, specific gene fragments were amplified by polymerase chain reaction, and PSGL-1 VNTR sequences were genotyped using an automatic nucleic acid analyzer. The correlations of PSGL-1 VNTR polymorphism with the susceptibility to EV-71-associated severe HFMD and the post-infection immune status were analyzed. The PSGL-1 VNTR A allele was identified as a susceptible SNP for severe HFMD. The risk of severe HFMD was higher for AA + AB genotype carriers than for BB genotype carriers. The counts of peripheral blood lymphocyte subsets were lower in AA + AB genotype carries than in BB genotype carries. In conclusion, PSGL-1 VNTR polymorphism is associated with the susceptibility to EV-71-induced severe HFMD and the immune status after infection. PSGL-1 VNTR might play a certain role in the pathogenesis of severe cases.
肠道病毒 71 型（EV-71）具有较强的嗜神经性，是引起重症手足口病（HFMD）的主要病原体。在临床观察中，EV-71 感染患儿的 HFMD 严重程度和预后存在显著差异。个体间的遗传差异可能是导致对 EV-71 诱导的 HFMD 易感性差异的重要原因之一。由于 P 选择素糖蛋白配体-1（PSGL-1）是 EV-71 的重要受体，因此研究 PSGL-1 单核苷酸多态性（SNP）与 EV-71 感染后重症 HFMD 的易感性之间的相关性是值得的。鉴于 PSGL-1 在免疫中的作用，研究 PSGL-1 SNP 与 EV-71 感染后免疫状态之间的相关性也是值得的。同时，PSGL-1 可变数串联重复（VNTR）是心血管和脑血管疾病的研究热点，但 EV-71 感染引起的 HFMD 尚未研究 PSGL-1 VNTR 多态性。在本研究中，通过聚合酶链反应扩增特定基因片段，并用自动核酸分析仪对 PSGL-1 VNTR 序列进行基因分型。分析 PSGL-1 VNTR 多态性与 EV-71 相关重症 HFMD 易感性及感染后免疫状态的相关性。PSGL-1 VNTR A 等位基因被鉴定为重症 HFMD 的易感 SNP。AA+AB 基因型携带者患重症 HFMD 的风险高于 BB 基因型携带者。AA+AB 基因型携带者的外周血淋巴细胞亚群计数低于 BB 基因型携带者。综上所述，PSGL-1 VNTR 多态性与 EV-71 诱导的重症 HFMD 易感性及感染后免疫状态相关。PSGL-1 VNTR 可能在重症病例的发病机制中起一定作用。
