Department of Otolaryngology-Head and Neck Surgery, QingPu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, People's Republic of China.
Ann Otol Rhinol Laryngol. 2024 Oct;133(10):886-892. doi: 10.1177/00034894241275449. Epub 2024 Aug 15.
Exposure to benzo[α]pyrene (BaP) increases the incidence and severity of allergic rhinitis (AR), but the underlying mechanisms remain unclear. Thus, we investigated the in vivo effects of BaP exposure on mucus hypersecretion and tissue remodeling in a rat model of AR.
Female Sprague-Dawley rats were randomly divided into 4 groups: a negative control group, a group of healthy rats exposed to BaP, a group of rats with ovalbumin (OVA)-induced AR, and a group of AR model rats exposed to BaP. Nasal symptoms and levels of OVA-specific serum immunoglobulin E (IgE) were measured in each individual rat. Moreover, examination of goblet cell hyperplasia and collagen deposition was carried out with periodic acid-Schiff (PAS) staining and Masson trichrome (MT) staining. Mucin 5AC (MUC5AC) expression was assessed by immunohistochemistry.
BaP significantly increased the number of sneezes, the number of nasal rubs and the levels of OVA-specific serum IgE in rats with AR. Statistically significant differences in goblet cell hyperplasia and collagen deposition were observed between the BaP-exposed AR model group and the AR model group. Immunohistochemical results showed that the nasal mucosa of AR model rats displayed markedly elevated MUC5AC expression after BaP exposure.
Our data indicate that mucus hypersecretion and the development of nasal remodeling might be pathophysiologic mechanisms underlying increased susceptibility to AR after exposure to BaP.
苯并[a]芘（BaP）暴露会增加变应性鼻炎（AR）的发病率和严重程度，但潜在机制尚不清楚。因此，我们研究了 BaP 暴露对 AR 大鼠模型中黏液高分泌和组织重塑的体内影响。
将雌性 Sprague-Dawley 大鼠随机分为 4 组：阴性对照组、BaP 暴露健康组、卵清蛋白（OVA）诱导的 AR 组和 AR 模型大鼠 BaP 暴露组。测量每只大鼠的鼻部症状和 OVA 特异性血清免疫球蛋白 E（IgE）水平。此外，通过过碘酸-Schiff（PAS）染色和 Masson 三色（MT）染色检查杯状细胞增生和胶原沉积。通过免疫组织化学评估粘蛋白 5AC（MUC5AC）的表达。
BaP 显著增加了 AR 大鼠的喷嚏次数、鼻擦次数和 OVA 特异性血清 IgE 水平。BaP 暴露的 AR 模型组与 AR 模型组之间在杯状细胞增生和胶原沉积方面存在统计学显著差异。免疫组织化学结果显示，AR 模型大鼠的鼻黏膜在 BaP 暴露后 MUC5AC 的表达明显升高。
我们的数据表明，黏液高分泌和鼻重塑的发展可能是 BaP 暴露后对 AR 易感性增加的病理生理机制。
