Yutani K, Ogasahara K, Sugino Y
Adv Biophys. 1985;20:13-29. doi: 10.1016/0065-227x(85)90028-0.
This paper reviews studies on thermostable proteins from thermophilic bacteria and on mutant proteins of human hemoglobin, tryptophan synthase alpha-subunit of E. coli, T4 phage lysozyme, and phage lambda repressor with respect to the role of the constituting amino acid residues in stabilization of conformation. The stability of a protein is easily affected by single amino acid substitutions, by which the protein undergoes change(s) of one or more of the following: a hydrogen bond, a salt bridge, a hydrophobic interaction, the volume of the residue, a disulfide bond, or the relative position of two aromatic rings.
本文综述了有关嗜热菌热稳定蛋白以及人血红蛋白、大肠杆菌色氨酸合成酶α亚基、T4噬菌体溶菌酶和λ噬菌体阻遏物的突变蛋白的研究，内容涉及构成氨基酸残基在构象稳定中的作用。蛋白质的稳定性很容易受到单个氨基酸取代的影响，通过这种取代，蛋白质会发生以下一种或多种变化：氢键、盐桥、疏水相互作用、残基体积、二硫键或两个芳香环的相对位置。
