Kong Xiaotian, Han Liang, Yang Liqin, Shi Zhifen, Lang Jiaqi, Ye Mingyan, Xiao Bo, Chen Xubing, Zhou Nong
College of Pharmacy, Dali University, Dali, 671000, China.
College of Food and Biological Engineering, Chongqing Three Gorges University, Chongqing, 404020, China.
Heliyon. 2024 Jul 17;10(14):e34846. doi: 10.1016/j.heliyon.2024.e34846. eCollection 2024 Jul 30.
. P. Y. Li represents a significant asset within traditional Chinese medicinal flora, though it confronts the challenge of germplasm deterioration during its cultivation phase. This study aimed to discern the implications of single strains or combinations of diverse growth-promoting actinomycetes on the growth metrics, antioxidant competence and pertinent gene expression in the leaves of . . The result revealed that the malondialdehyde content within the plant's leaves notably diminished in the treatment groups compared to the CK group, with the S6 group showcasing the most pronounced malondialdehyde reduction, amounting to approximately one-third of the CK's value. Leaf area, length and width peaked in the S5 cohort, registering values 4.55, 2.46 and 1.85 times surpassing the CK group. Concurrently, plant height and stem thickness were maximal in the S6 group, being 2.29 and 1.75 times that of the CK group, whereas leaf thickness reached its zenith in the S7 group, marking a 2.17-fold elevation compared to the CK. Photosynthetic pigments, soluble sugars and soluble proteins in the leaves, exhibited augmentation across the inoculated groups to varying magnitudes. Specifically, the S5 group was superior in photosynthetic metrics and pigments, while the S6 group manifested the highest soluble sugar concentration, which was 1.35 times that of the CK. The S3 group demonstrated the pinnacle of soluble protein content, an impressive 5.86-fold increment relative to the CK group. The enzymatic activities of superoxide dismutase, peroxidase and catalase, along with their affiliated gene expressions, were observably augmented in the inoculated groups, with the S5 group standing out. To encapsulate, the actinomycete inoculation holds potential in fostering the growth and maturation of . , amplifying its environmental resilience. The revelations from this study extend valuable insights for the judicious choice of microbial fertilizers in the cultivated propagation of P. Y. Li.
李叶绣线菊是中国传统药用植物中的重要资源，但其在栽培过程中面临种质退化的挑战。本研究旨在探讨单一菌株或多种促生长放线菌组合对李叶绣线菊叶片生长指标、抗氧化能力及相关基因表达的影响。结果表明，与对照（CK）组相比，处理组植物叶片中的丙二醛含量显著降低，其中S6组丙二醛含量降低最为明显，约为CK组的三分之一。叶面积、叶长和叶宽在S5组达到峰值，分别是CK组的4.55倍、2.46倍和1.85倍。同时，株高和茎粗在S6组最大，分别是CK组的2.29倍和1.75倍，而叶片厚度在S7组达到最大值，比CK组高2.17倍。接种组叶片中的光合色素、可溶性糖和可溶性蛋白均有不同程度的增加。具体而言，S5组在光合指标和色素方面表现优异，而S6组可溶性糖浓度最高，是CK组的1.35倍。S3组可溶性蛋白含量最高，相对于CK组增加了5.86倍。接种组超氧化物歧化酶、过氧化物酶和过氧化氢酶的酶活性及其相关基因表达均显著增加，其中S5组最为突出。综上所述，接种放线菌具有促进李叶绣线菊生长和成熟、增强其环境适应能力的潜力。本研究结果为李叶绣线菊栽培繁殖中微生物肥料的合理选择提供了有价值的参考。
