Weise Chris, Schirmer Martin, Polack Matthias, Korell Alexander, Westphal Hannes, Schwieger Julius, Warias Rico, Zimmermann Stefan, Belder Detlev
University Leipzig, Linnestrasse 3, Leipzig 04103, Germany.
UFZ Leipzig, Permoserstrasse 15, Leipzig 04318, Germany.
Anal Chem. 2024 Aug 17;96(34):13888-96. doi: 10.1021/acs.analchem.4c01958.
This study presents the development of a miniaturized device for supercritical fluid chromatography coupled with mass spectrometry. The chip-based, modular nanoSFC approach utilizes a particle-packed nanobore column embedded between two monolithically structured glass chips. A microtee in the pre-column section ensures picoliter sample loads onto the column, while a microcross chip structure fluidically controls the column backpressure. The restrictive emitter and the minimal post-column volume of 16 nL prevent mobile phase decompression and analyte dilution, maintaining chromatographic integrity during transfer to the atmospheric pressure MS interface. This facilitates high-speed chiral separations in less than 80 s with high reproducibility.
本研究介绍了一种用于超临界流体色谱与质谱联用的小型化装置的开发。基于芯片的模块化纳米超临界流体色谱方法利用了填充颗粒的纳米孔柱，该柱嵌入在两个整体结构的玻璃芯片之间。柱前部分的微三通确保将皮升级别的样品加载到柱上，而微交叉芯片结构以流体方式控制柱背压。限流发射器和16 nL的最小柱后体积可防止流动相减压和分析物稀释，在转移至大气压质谱接口的过程中保持色谱完整性。这有助于在不到80秒的时间内实现高速手性分离，且具有高重现性。
