Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, 76169-13555 Kerman, Iran; Department of Chemistry, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr 75168, Iran.
Int J Biol Macromol. 2024 Oct;278(Pt 2):134813. doi: 10.1016/j.ijbiomac.2024.134813. Epub 2024 Aug 21.
With rapid industrial expansion, environmental pollution from emerging contaminants has increased, posing severe ecosystem threats. Laccases offer an eco-friendly solution for degrading hazardous substances, but their use as free-form biocatalysts face challenges. This study immobilized laccase (PersiLac1) on green-synthesized Si@Fe nanoparticles (MSFM NPs) to remove pollutants like Malachite Green-containing wastewater and degrade plastic films. Characterization techniques (FTIR, VSM, XRD, SEM, EDS, BET) confirmed the properties and structure of MSFM NPs, revealing a surface area of 31.297 m.g and a pore diameter of 12.267 nm. The immobilized PersiLac1 showed enhanced activity across various temperatures and pH levels, retaining over 82 % activity after 15 cycles at 80°C with minimal leaching. It demonstrated higher stability, half-life, and decimal reduction time than free laccase. Under 1 M NaCl, its activity was 1.8 times higher than the non-immobilized enzyme. The immobilized laccase removed 98.11 % of Malachite Green-containing wastewater and retained 82.92 % activity over twenty cycles of dye removal. Additionally, FTIR and SEM confirmed superior plastic degradation under saline conditions. These findings suggest that immobilizing PersiLac1 on magnetic nanoparticles enhances its function and potential for contaminant removal. Future research should focus on scalable, cost-effective laccase immobilization methods for large-scale environmental applications.
随着工业的快速扩张，新兴污染物造成的环境污染不断增加，对生态系统构成了严重威胁。漆酶为降解有害物质提供了一种环保的解决方案，但作为游离形式的生物催化剂，其应用面临挑战。本研究将漆酶（PersiLac1）固定在绿色合成的 Si@Fe 纳米粒子（MSFM NPs）上，以去除含有孔雀石绿的废水和降解塑料薄膜等污染物。表征技术（FTIR、VSM、XRD、SEM、EDS、BET）证实了 MSFM NPs 的性质和结构，其比表面积为 31.297 m.g，孔径为 12.267 nm。固定化的 PersiLac1 在各种温度和 pH 值下表现出增强的活性，在 80°C 下经过 15 次循环后，仍保留超过 82%的活性，且最小化了酶的泄露。与游离酶相比，它表现出更高的稳定性、半衰期和十进制减少时间。在 1 M NaCl 下，其活性是未固定化酶的 1.8 倍。固定化漆酶去除了 98.11%的含有孔雀石绿的废水，在 20 次染料去除循环中保留了 82.92%的活性。此外，FTIR 和 SEM 证实了在盐度条件下具有优越的塑料降解能力。这些发现表明，将 PersiLac1 固定在磁性纳米粒子上可以增强其功能和去除污染物的潜力。未来的研究应集中在可扩展、具有成本效益的大规模环境应用的漆酶固定化方法上。
